Project name: SH3_E107P

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	EA107P
Energy difference between WT (input) and mutated protein (by FoldX)	8.28555 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1049
• Maximal score value: 1.8034
• Average score: -0.9629
• Total score value: -54.8853

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5161
86	L	A	0.7962
87	F	A	0.9638
88	V	A	0.5122
89	A	A	0.0000
90	L	A	-0.0944
91	Y	A	-0.5154
92	D	A	-2.4735
93	Y	A	-1.8742
94	E	A	-2.6064
95	A	A	-2.6014
96	R	A	-2.9879
97	T	A	-2.6628
98	E	A	-3.1049
99	D	A	-3.0452
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.1297
103	F	A	-1.7443
104	H	A	-2.5676
105	K	A	-2.2834
106	G	A	-1.2993
107	P	A	-1.0402	mutated: EA107P
108	K	A	-0.4927
109	F	A	0.0000
110	Q	A	-0.5064
111	I	A	-0.0543
112	L	A	0.1430
113	N	A	-0.8879
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0557
121	E	A	-1.1529
122	A	A	0.0000
123	R	A	-1.7144
124	S	A	0.0000
125	L	A	0.0974
126	T	A	-0.3988
127	T	A	-0.7788
128	G	A	-1.3607
129	E	A	-2.2237
130	T	A	-1.6955
131	G	A	-1.5015
132	Y	A	-0.8695
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1508
137	Y	A	-0.1056
138	V	A	0.0000
139	A	A	0.4213
140	P	A	0.7784
141	V	A	1.8034