Project name: SH3_E121G

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	EA121G
Energy difference between WT (input) and mutated protein (by FoldX)	1.67182 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.0871
• Maximal score value: 1.7964
• Average score: -0.9117
• Total score value: -51.9669

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5140
86	L	A	0.7869
87	F	A	0.9198
88	V	A	0.4286
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6087
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.0871
99	D	A	-2.9544
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.1901
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3078
108	K	A	-0.6465
109	F	A	0.0000
110	Q	A	-0.4155
111	I	A	0.1554
112	L	A	0.4974
113	N	A	-0.8440
114	S	A	-1.1307
115	S	A	-1.5953
116	E	A	-2.5697
117	G	A	-2.1306
118	D	A	-2.4136
119	W	A	-1.0055
120	W	A	-0.8131
121	G	A	-0.6324	mutated: EA121G
122	A	A	0.0000
123	R	A	-1.5409
124	S	A	0.0000
125	L	A	0.0565
126	T	A	-0.4705
127	T	A	-0.8174
128	G	A	-1.3532
129	E	A	-2.2418
130	T	A	-1.5276
131	G	A	-1.2476
132	Y	A	-0.5428
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.8464
136	N	A	-1.1519
137	Y	A	-0.1254
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7735
141	V	A	1.7964