Project name: SH3_R96T

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	RA96T
Energy difference between WT (input) and mutated protein (by FoldX)	1.4499 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -2.836
• Maximal score value: 1.7964
• Average score: -0.8995
• Total score value: -51.2703

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9235
88	V	A	0.4306
89	A	A	0.0000
90	L	A	-0.1469
91	Y	A	-0.5656
92	D	A	-2.5420
93	Y	A	-1.7111
94	E	A	-2.2268
95	A	A	-1.8006
96	T	A	-1.2513	mutated: RA96T
97	T	A	-1.8555
98	E	A	-2.7906
99	D	A	-2.8360
100	D	A	-1.7963
101	L	A	0.0000
102	S	A	-1.9625
103	F	A	0.0000
104	H	A	-2.7196
105	K	A	-2.4002
106	G	A	-1.4592
107	E	A	-1.3102
108	K	A	-0.6438
109	F	A	0.0000
110	Q	A	-0.5091
111	I	A	-0.0572
112	L	A	0.1426
113	N	A	-0.8653
114	S	A	-1.1456
115	S	A	-1.5762
116	E	A	-2.5300
117	G	A	-2.0899
118	D	A	-2.3656
119	W	A	-0.9646
120	W	A	-0.9961
121	E	A	-1.1421
122	A	A	0.0000
123	R	A	-1.7160
124	S	A	0.0000
125	L	A	0.0587
126	T	A	-0.4736
127	T	A	-0.8209
128	G	A	-1.3572
129	E	A	-2.2402
130	T	A	-1.7199
131	G	A	-1.5449
132	Y	A	-0.8989
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.8674
136	N	A	-1.1252
137	Y	A	-0.1001
138	V	A	0.0000
139	A	A	0.4159
140	P	A	0.7757
141	V	A	1.7964