Project name: SH3_R123P

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	RA123P
Energy difference between WT (input) and mutated protein (by FoldX)	3.92709 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1029
• Maximal score value: 1.762
• Average score: -0.8883
• Total score value: -50.6342

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.3443
86	L	A	0.5997
87	F	A	0.7327
88	V	A	0.3411
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5597
93	Y	A	-1.9312
94	E	A	-2.6448
95	A	A	-2.6256
96	R	A	-2.9850
97	T	A	-2.6607
98	E	A	-3.1029
99	D	A	-3.0566
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2073
103	F	A	0.0000
104	H	A	-2.7280
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.2992
108	K	A	-0.6065
109	F	A	0.0000
110	Q	A	-0.5658
111	I	A	0.2442
112	L	A	0.9729
113	N	A	-0.6077
114	S	A	-1.0303
115	S	A	-1.5116
116	E	A	-2.5707
117	G	A	-2.1420
118	D	A	-2.4530
119	W	A	-1.1207
120	W	A	-0.8787
121	E	A	-0.8292
122	A	A	0.0000
123	P	A	-0.6851	mutated: RA123P
124	S	A	0.0000
125	L	A	0.2094
126	T	A	-0.3332
127	T	A	-0.7062
128	G	A	-1.2159
129	E	A	-1.9555
130	T	A	-1.2353
131	G	A	-1.2124
132	Y	A	-0.6685
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9349
136	N	A	-1.1504
137	Y	A	-0.1243
138	V	A	0.0000
139	A	A	0.3992
140	P	A	0.6891
141	V	A	1.7620