Project name: SH3_A95T

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	AA95T
Energy difference between WT (input) and mutated protein (by FoldX)	5.89415 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1045
• Maximal score value: 1.7964
• Average score: -0.9594
• Total score value: -54.6846

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9237
88	V	A	0.4309
89	A	A	0.0000
90	L	A	-0.1503
91	Y	A	-0.5733
92	D	A	-2.5550
93	Y	A	-1.9235
94	E	A	-2.6441
95	T	A	-2.6392	mutated: AA95T
96	R	A	-2.9930
97	T	A	-2.6651
98	E	A	-3.1045
99	D	A	-3.0448
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.1872
103	F	A	0.0000
104	H	A	-2.7221
105	K	A	-2.4055
106	G	A	-1.4592
107	E	A	-1.3102
108	K	A	-0.6438
109	F	A	0.0000
110	Q	A	-0.5091
111	I	A	-0.0568
112	L	A	0.1429
113	N	A	-0.8876
114	S	A	-1.1788
115	S	A	-1.5959
116	E	A	-2.5594
117	G	A	-2.1332
118	D	A	-2.4432
119	W	A	-1.1000
120	W	A	-1.0544
121	E	A	-1.1524
122	A	A	0.0000
123	R	A	-1.7155
124	S	A	0.0000
125	L	A	0.0587
126	T	A	-0.4736
127	T	A	-0.8209
128	G	A	-1.3572
129	E	A	-2.2369
130	T	A	-1.6899
131	G	A	-1.4979
132	Y	A	-0.8664
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9192
136	N	A	-1.1492
137	Y	A	-0.1213
138	V	A	0.0000
139	A	A	0.4162
140	P	A	0.7757
141	V	A	1.7964