Project name: SH3_L125G

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	LA125G
Energy difference between WT (input) and mutated protein (by FoldX)	2.33442 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.8882
• Average score: -1.0722
• Total score value: -61.1172

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.6925
86	L	A	0.9080
87	F	A	0.8311
88	V	A	0.1761
89	A	A	0.0000
90	L	A	-0.1951
91	Y	A	-0.5854
92	D	A	-2.5609
93	Y	A	-1.9321
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7625
105	K	A	-2.4498
106	G	A	-1.5349
107	E	A	-1.7617
108	K	A	-1.3353
109	F	A	0.0000
110	Q	A	-0.9735
111	I	A	-0.1005
112	L	A	0.0781
113	N	A	-0.9146
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0831
121	E	A	-1.1919
122	A	A	0.0000
123	R	A	-2.2115
124	S	A	0.0000
125	G	A	-1.5979	mutated: LA125G
126	T	A	-1.3116
127	T	A	-1.3061
128	G	A	-1.8753
129	E	A	-2.5054
130	T	A	-1.9109
131	G	A	-1.5075
132	Y	A	-0.8724
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1511
137	Y	A	-0.1361
138	V	A	0.0000
139	A	A	0.4388
140	P	A	0.8436
141	V	A	1.8882