Project name: SH3_D99G

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	DA99G
Energy difference between WT (input) and mutated protein (by FoldX)	0.239361 kcal/mol Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -2.736
• Maximal score value: 1.7964
• Average score: -0.8713
• Total score value: -49.6633

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9238
88	V	A	0.4313
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.3610
96	R	A	-2.7360
97	T	A	-2.1778
98	E	A	-2.3635
99	G	A	-1.5299	mutated: DA99G
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.0328
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3078
108	K	A	-0.6426
109	F	A	0.0000
110	Q	A	-0.5065
111	I	A	-0.0382
112	L	A	0.1674
113	N	A	-0.8696
114	S	A	-1.1675
115	S	A	-1.5970
116	E	A	-2.5631
117	G	A	-2.1386
118	D	A	-2.4379
119	W	A	-1.0813
120	W	A	-1.0207
121	E	A	-0.9311
122	A	A	0.0000
123	R	A	-1.6967
124	S	A	0.0000
125	L	A	0.0591
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3571
129	E	A	-2.2399
130	T	A	-1.4709
131	G	A	-0.9913
132	Y	A	-0.3485
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9090
136	N	A	-1.1527
137	Y	A	-0.1259
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7757
141	V	A	1.7964