Project name: SH3_T130L

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	TA130L
Energy difference between WT (input) and mutated protein (by FoldX)	-1.20412 kcal/mol Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.103
• Maximal score value: 1.7964
• Average score: -0.8872
• Total score value: -50.5728

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.4749
86	L	A	0.7229
87	F	A	0.8751
88	V	A	0.4066
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6265
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1030
99	D	A	-2.9104
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.1047
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3042
108	K	A	-0.6781
109	F	A	0.0000
110	Q	A	-0.5568
111	I	A	0.0070
112	L	A	0.3041
113	N	A	-0.8248
114	S	A	-1.1344
115	S	A	-1.5705
116	E	A	-2.5402
117	G	A	-2.1205
118	D	A	-2.4322
119	W	A	-1.0807
120	W	A	-1.0206
121	E	A	-0.8713
122	A	A	0.0000
123	R	A	-1.2758
124	S	A	0.0000
125	L	A	0.1785
126	T	A	-0.4304
127	T	A	-0.6250
128	G	A	-1.0105
129	E	A	-1.6886
130	L	A	-0.6925	mutated: TA130L
131	G	A	-0.9855
132	Y	A	-0.6339
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9129
136	N	A	-1.1500
137	Y	A	-0.1241
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7547
141	V	A	1.7964