Project name: SH3_Y132W

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	YA132W
Energy difference between WT (input) and mutated protein (by FoldX)	1.52782 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.0776
• Maximal score value: 1.7964
• Average score: -0.9205
• Total score value: -52.4707

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5183
86	L	A	0.7945
87	F	A	0.9250
88	V	A	0.4313
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5596
93	Y	A	-1.9310
94	E	A	-2.6446
95	A	A	-2.6019
96	R	A	-2.9848
97	T	A	-2.6606
98	E	A	-3.0776
99	D	A	-2.9857
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.1804
103	F	A	0.0000
104	H	A	-2.7280
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3078
108	K	A	-0.6420
109	F	A	0.0000
110	Q	A	-0.5073
111	I	A	-0.0335
112	L	A	0.1498
113	N	A	-0.8672
114	S	A	-1.1633
115	S	A	-1.5964
116	E	A	-2.5557
117	G	A	-2.1336
118	D	A	-2.4315
119	W	A	-1.0611
120	W	A	-0.9897
121	E	A	-1.0402
122	A	A	0.0000
123	R	A	-1.6806
124	S	A	0.0000
125	L	A	0.0590
126	T	A	-0.4730
127	T	A	-0.8208
128	G	A	-1.3523
129	E	A	-2.2289
130	T	A	-1.6331
131	G	A	0.0000
132	W	A	-0.6944	mutated: YA132W
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9008
136	N	A	-1.1530
137	Y	A	-0.1262
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7757
141	V	A	1.7964