Project name: SH3_H104M

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	HA104M
Energy difference between WT (input) and mutated protein (by FoldX)	1.00571 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1005
• Maximal score value: 1.7964
• Average score: -0.8194
• Total score value: -46.7075

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9270
88	V	A	0.6700
89	A	A	0.0000
90	L	A	0.1627
91	Y	A	-0.0616
92	D	A	-1.6340
93	Y	A	-1.4195
94	E	A	-2.3405
95	A	A	-2.6202
96	R	A	-2.9831
97	T	A	-2.6585
98	E	A	-3.1005
99	D	A	-3.0413
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-1.7762
103	F	A	0.0000
104	M	A	-0.6765	mutated: HA104M
105	K	A	-1.3991
106	G	A	-0.9051
107	E	A	-0.6625
108	K	A	-0.3836
109	F	A	0.0000
110	Q	A	-0.5091
111	I	A	-0.0568
112	L	A	0.1429
113	N	A	-0.8879
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0557
121	E	A	-1.1530
122	A	A	0.0000
123	R	A	-1.7155
124	S	A	0.0000
125	L	A	0.0635
126	T	A	-0.2534
127	T	A	-0.8183
128	G	A	-1.3572
129	E	A	-2.2377
130	T	A	-1.6906
131	G	A	-1.4963
132	Y	A	-0.8647
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1504
137	Y	A	-0.1259
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7757
141	V	A	1.7964