Project name: SH3_V88F

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	VA88F
Energy difference between WT (input) and mutated protein (by FoldX)	1.9635 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.8377
• Average score: -0.9532
• Total score value: -54.3325

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5310
86	L	A	0.8299
87	F	A	0.9853
88	F	A	0.5531	mutated: VA88F
89	A	A	0.0000
90	L	A	-0.2378
91	Y	A	-0.6157
92	D	A	-2.5850
93	Y	A	-1.9464
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7302
105	K	A	-2.4201
106	G	A	-1.4436
107	E	A	-1.2709
108	K	A	-0.5785
109	F	A	0.0000
110	Q	A	-0.4819
111	I	A	-0.0574
112	L	A	0.1430
113	N	A	-0.8879
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1026
120	W	A	-1.0557
121	E	A	-1.1529
122	A	A	0.0000
123	R	A	-1.7052
124	S	A	0.0000
125	L	A	0.1166
126	T	A	-0.4524
127	T	A	-0.8087
128	G	A	-1.3446
129	E	A	-2.2334
130	T	A	-1.6879
131	G	A	-1.4991
132	Y	A	-0.8673
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9204
136	N	A	-1.1676
137	Y	A	-0.1393
138	V	A	0.0000
139	A	A	0.4338
140	P	A	0.8127
141	V	A	1.8377