Project name: SH3_V88C

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	VA88C
Energy difference between WT (input) and mutated protein (by FoldX)	1.22601 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7408
• Average score: -0.9681
• Total score value: -55.1802

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.4924
86	L	A	0.7568
87	F	A	0.8377
88	C	A	0.2432	mutated: VA88C
89	A	A	0.0000
90	L	A	-0.1552
91	Y	A	-0.5792
92	D	A	-2.5481
93	Y	A	-1.9262
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7360
105	K	A	-2.4177
106	G	A	-1.4851
107	E	A	-1.3284
108	K	A	-0.6625
109	F	A	0.0000
110	Q	A	-0.5190
111	I	A	-0.0509
112	L	A	0.1430
113	N	A	-0.8879
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0557
121	E	A	-1.1529
122	A	A	0.0000
123	R	A	-1.6964
124	S	A	0.0000
125	L	A	0.0938
126	T	A	-0.4374
127	T	A	-0.8026
128	G	A	-1.3446
129	E	A	-2.2334
130	T	A	-1.6879
131	G	A	-1.4991
132	Y	A	-0.8673
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9202
136	N	A	-1.1439
137	Y	A	-0.1464
138	V	A	0.0000
139	A	A	0.3601
140	P	A	0.7219
141	V	A	1.7408