Project name: SH3_F87G

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	FA87G
Energy difference between WT (input) and mutated protein (by FoldX)	5.72231 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.745
• Average score: -0.9576
• Total score value: -54.5807

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.4865
86	L	A	0.7374
87	G	A	0.0000	mutated: FA87G
88	V	A	0.3819
89	A	A	0.0000
90	L	A	-0.1221
91	Y	A	-0.5459
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.3827
106	G	A	-1.4635
107	E	A	-1.3212
108	K	A	-0.6756
109	F	A	0.0000
110	Q	A	-0.4991
111	I	A	0.0278
112	L	A	0.2155
113	N	A	-0.8211
114	S	A	-1.1115
115	S	A	-1.5747
116	E	A	-2.5353
117	G	A	-2.1083
118	D	A	-2.4105
119	W	A	-1.0417
120	W	A	-0.9111
121	E	A	-1.0662
122	A	A	0.0000
123	R	A	-1.6770
124	S	A	0.0000
125	L	A	0.0372
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6812
131	G	A	-1.4687
132	Y	A	-0.8153
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.8509
136	N	A	-1.0835
137	Y	A	-0.0665
138	V	A	0.2571
139	A	A	0.4252
140	P	A	0.7191
141	V	A	1.7450