Project name: SH3_T130Y

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	TA130Y
Energy difference between WT (input) and mutated protein (by FoldX)	-0.40078 kcal/mol Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1019
• Maximal score value: 1.7964
• Average score: -0.8369
• Total score value: -47.7011

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.4749
86	L	A	0.7431
87	F	A	0.8751
88	V	A	0.4066
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6252
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1019
99	D	A	-2.8620
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.0473
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3042
108	K	A	-0.6487
109	F	A	0.0000
110	Q	A	-0.4269
111	I	A	0.1363
112	L	A	0.4822
113	N	A	-0.8266
114	S	A	-1.1395
115	S	A	-1.5752
116	E	A	-2.5584
117	G	A	-2.1329
118	D	A	-2.4432
119	W	A	-1.0996
120	W	A	-1.0138
121	E	A	-0.7697
122	A	A	0.0000
123	R	A	-0.8476
124	S	A	0.0000
125	L	A	0.2922
126	T	A	-0.3820
127	T	A	-0.4987
128	G	A	-0.7871
129	E	A	-1.3039
130	Y	A	-0.1403	mutated: TA130Y
131	G	A	-0.7180
132	Y	A	-0.5311
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9192
136	N	A	-1.1504
137	Y	A	-0.1243
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7547
141	V	A	1.7964