Project name: SH3_L86Y

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	LA86Y
Energy difference between WT (input) and mutated protein (by FoldX)	0.711985 kcal/mol Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.8193
• Average score: -1.0141
• Total score value: -57.8052

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.4978
86	Y	A	0.6910	mutated: LA86Y
87	F	A	0.8634
88	V	A	0.3828
89	A	A	0.0000
90	L	A	-0.1547
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7323
105	K	A	-2.4106
106	G	A	-1.4668
107	E	A	-1.3607
108	K	A	-0.8521
109	F	A	0.0000
110	Q	A	-0.9265
111	I	A	-0.2212
112	L	A	0.2062
113	N	A	-0.8066
114	S	A	-1.1309
115	S	A	-1.5656
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0972
121	E	A	-1.2243
122	A	A	0.0000
123	R	A	-2.3299
124	S	A	0.0000
125	L	A	-0.3070
126	T	A	-0.6421
127	T	A	-0.9591
128	G	A	-1.6036
129	E	A	-2.4355
130	T	A	-1.9484
131	G	A	-1.5935
132	Y	A	-0.9160
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9252
136	N	A	-1.1504
137	Y	A	-0.1243
138	V	A	0.0000
139	A	A	0.4256
140	P	A	0.7697
141	V	A	1.8193