Project name: SH3_V88D

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	VA88D
Energy difference between WT (input) and mutated protein (by FoldX)	4.15849 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.5784
• Average score: -1.017
• Total score value: -57.9678

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.4354
86	L	A	0.6223
87	F	A	0.5537
88	D	A	-0.3261	mutated: VA88D
89	A	A	0.0000
90	L	A	-0.3072
91	Y	A	-0.6516
92	D	A	-2.5480
93	Y	A	-1.9278
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7997
105	K	A	-2.5222
106	G	A	-1.6492
107	E	A	-1.5085
108	K	A	-0.9251
109	F	A	0.0000
110	Q	A	-0.5965
111	I	A	-0.0535
112	L	A	0.1430
113	N	A	-0.8877
114	S	A	-1.1790
115	S	A	-1.5957
116	E	A	-2.5591
117	G	A	-2.1323
118	D	A	-2.4437
119	W	A	-1.1023
120	W	A	-1.0554
121	E	A	-1.1528
122	A	A	0.0000
123	R	A	-1.6998
124	S	A	0.0000
125	L	A	0.0250
126	T	A	-0.4406
127	T	A	-0.8050
128	G	A	-1.3446
129	E	A	-2.2334
130	T	A	-1.6879
131	G	A	-1.4991
132	Y	A	-0.8673
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9202
136	N	A	-1.1458
137	Y	A	-0.2373
138	V	A	0.0000
139	A	A	0.1753
140	P	A	0.5727
141	V	A	1.5784