Project name: SH3_F87L

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	FA87L
Energy difference between WT (input) and mutated protein (by FoldX)	2.04795 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7178
• Average score: -0.9868
• Total score value: -56.2453

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.4655
86	L	A	0.6850
87	L	A	0.0000	mutated: FA87L
88	V	A	0.3188
89	A	A	0.0000
90	L	A	-0.1780
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4855
107	E	A	-1.3428
108	K	A	-0.7127
109	F	A	0.0000
110	Q	A	-0.5525
111	I	A	-0.0735
112	L	A	0.1605
113	N	A	-0.8695
114	S	A	-1.1585
115	S	A	-1.5896
116	E	A	-2.5542
117	G	A	-2.1315
118	D	A	-2.4360
119	W	A	-1.0846
120	W	A	-1.0129
121	E	A	-1.1288
122	A	A	0.0000
123	R	A	-1.7070
124	S	A	0.0000
125	L	A	0.0353
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6906
131	G	A	-1.4909
132	Y	A	-0.8519
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9357
136	N	A	-1.1458
137	Y	A	-0.1504
138	V	A	0.0000
139	A	A	0.3387
140	P	A	0.6765
141	V	A	1.7178