Project name: SH3_L86L

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	LA86L
Energy difference between WT (input) and mutated protein (by FoldX)	1.27898e-12 kcal/mol Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.784
• Average score: -0.9631
• Total score value: -54.8942

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.4955
86	L	A	0.7509	mutated: LA86L
87	F	A	0.9010
88	V	A	0.4194
89	A	A	0.0000
90	L	A	-0.1520
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7292
105	K	A	-2.4071
106	G	A	-1.4602
107	E	A	-1.3070
108	K	A	-0.6566
109	F	A	0.0000
110	Q	A	-0.5398
111	I	A	-0.0759
112	L	A	0.1378
113	N	A	-0.8903
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0578
121	E	A	-1.1558
122	A	A	0.0000
123	R	A	-1.7213
124	S	A	0.0000
125	L	A	0.0711
126	T	A	-0.4632
127	T	A	-0.8147
128	G	A	-1.3523
129	E	A	-2.2362
130	T	A	-1.6924
131	G	A	-1.4991
132	Y	A	-0.8673
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1504
137	Y	A	-0.1243
138	V	A	0.0000
139	A	A	0.4095
140	P	A	0.7622
141	V	A	1.7840