| Chain sequence(s) |
A: MRSGSHHHHHHRSDITSLYKKAGSAAAPFTMENLYFQSYQGNSDCYFGNGSAYRGTHSLTESGASCLPWNSRILIGKVYTAQNPSAQALGLGKHNYCRNPDGDAKPWCHVLKNRRLTWEYCDVPSCSTCGLRQYSQPQFRIKGGLFADIASHPWQAAIFAKHRRSPGERFLCGGILISSCWILSAAHCFQERFPPHHLTVILGRTYRRVPGEEEQKFEVEKYIVHKEFDDDTYDNDIALLQLKSDSSRCAQESSVVRRVCLPPADLQLPDWTECELSGYGKHEALSPFYSERLKEAHVRLYPSSRCTSQHLLNRTVTDNMLCAGDTRSGGPQANLHDACQGDSGGPLVCLNDGRMTLVGIISWGLGCGQKDVPGVYTKVTNYLDWIRDNMR |
| Distance of aggregation | 10 Å |
| Dynamic mode | No |
| Mutated residues | RA258N |
| Energy difference between WT (input) and mutated protein (by FoldX) | -0.589191 kcal/mol
Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive. |
The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.
| residue index | residue name | chain | Aggrescan3D score | mutation |
|---|---|---|---|---|
| residue index | residue name | chain | Aggrescan3D score | |
| 1 | M | A | -0.0867 | |
| 2 | R | A | -1.5465 | |
| 3 | S | A | -0.6555 | |
| 4 | G | A | -0.5220 | |
| 5 | S | A | -0.3381 | |
| 6 | H | A | 0.0000 | |
| 7 | H | A | -0.5510 | |
| 8 | H | A | -0.7154 | |
| 9 | H | A | -1.1349 | |
| 10 | H | A | -1.8444 | |
| 11 | H | A | -1.6270 | |
| 12 | R | A | -1.8076 | |
| 13 | S | A | 0.0000 | |
| 14 | D | A | 0.0000 | |
| 15 | I | A | 0.6114 | |
| 16 | T | A | -0.0757 | |
| 17 | S | A | 0.0222 | |
| 18 | L | A | 0.1152 | |
| 19 | Y | A | 0.0000 | |
| 20 | K | A | -1.5965 | |
| 21 | K | A | -2.2823 | |
| 22 | A | A | -1.2274 | |
| 23 | G | A | -1.3144 | |
| 24 | S | A | -0.9120 | |
| 25 | A | A | 0.0000 | |
| 26 | A | A | -0.0740 | |
| 27 | A | A | 0.3831 | |
| 28 | P | A | -0.0164 | |
| 29 | F | A | 0.0000 | |
| 30 | T | A | -0.6249 | |
| 31 | M | A | -0.5325 | |
| 32 | E | A | -1.9905 | |
| 33 | N | A | -1.3394 | |
| 34 | L | A | 0.1316 | |
| 35 | Y | A | 0.8240 | |
| 36 | F | A | 0.1334 | |
| 37 | Q | A | -0.9129 | |
| 38 | S | A | -1.0619 | |
| 39 | Y | A | 0.0000 | |
| 40 | Q | A | 0.0000 | |
| 41 | G | A | -0.6844 | |
| 42 | N | A | -0.9168 | |
| 43 | S | A | 0.0000 | |
| 44 | D | A | -0.3403 | |
| 45 | C | A | 0.3624 | |
| 46 | Y | A | 1.0810 | |
| 47 | F | A | 0.0000 | |
| 48 | G | A | 0.0000 | |
| 49 | N | A | 0.0000 | |
| 50 | G | A | 0.0000 | |
| 51 | S | A | 0.0000 | |
| 52 | A | A | -0.9919 | |
| 53 | Y | A | 0.0000 | |
| 54 | R | A | -0.8030 | |
| 55 | G | A | -0.6611 | |
| 56 | T | A | -0.3863 | |
| 57 | H | A | -0.2321 | |
| 58 | S | A | -0.1056 | |
| 59 | L | A | -0.2649 | |
| 60 | T | A | 0.0000 | |
| 61 | E | A | -2.2355 | |
| 62 | S | A | -1.3994 | |
| 63 | G | A | -1.1712 | |
| 64 | A | A | -0.8180 | |
| 65 | S | A | -0.2706 | |
| 66 | C | A | 0.0000 | |
| 67 | L | A | -0.0092 | |
| 68 | P | A | -0.8655 | |
| 69 | W | A | 0.0000 | |
| 70 | N | A | -1.9564 | |
| 71 | S | A | -1.0866 | |
| 72 | R | A | -1.3915 | |
| 73 | I | A | 0.5659 | |
| 74 | L | A | 0.0000 | |
| 75 | I | A | -0.5193 | |
| 76 | G | A | -0.7421 | |
| 77 | K | A | -1.0308 | |
| 78 | V | A | 0.3291 | |
| 79 | Y | A | 0.9142 | |
| 80 | T | A | 0.3819 | |
| 81 | A | A | -0.0276 | |
| 82 | Q | A | -0.3391 | |
| 83 | N | A | -0.5037 | |
| 84 | P | A | -0.5208 | |
| 85 | S | A | -0.9529 | |
| 86 | A | A | 0.0000 | |
| 87 | Q | A | 0.0000 | |
| 88 | A | A | 0.0000 | |
| 89 | L | A | 0.0000 | |
| 90 | G | A | 0.0000 | |
| 91 | L | A | 0.0000 | |
| 92 | G | A | -1.5666 | |
| 93 | K | A | -2.3754 | |
| 94 | H | A | -1.3837 | |
| 95 | N | A | 0.0000 | |
| 96 | Y | A | -0.2150 | |
| 97 | C | A | 0.0000 | |
| 98 | R | A | 0.0000 | |
| 99 | N | A | 0.0000 | |
| 100 | P | A | -1.1536 | |
| 101 | D | A | -2.0428 | |
| 102 | G | A | -1.6743 | |
| 103 | D | A | -1.3105 | |
| 104 | A | A | -0.8200 | |
| 105 | K | A | -1.0048 | |
| 106 | P | A | 0.0000 | |
| 107 | W | A | -0.2389 | |
| 108 | C | A | 0.0000 | |
| 109 | H | A | 0.0000 | |
| 110 | V | A | 0.0000 | |
| 111 | L | A | -1.2644 | |
| 112 | K | A | -2.7717 | |
| 113 | N | A | -3.1772 | |
| 114 | R | A | -3.5318 | |
| 115 | R | A | -3.0852 | |
| 116 | L | A | -1.2189 | |
| 117 | T | A | -0.7363 | |
| 118 | W | A | 0.0180 | |
| 119 | E | A | -0.8730 | |
| 120 | Y | A | -0.4243 | |
| 121 | C | A | 0.0000 | |
| 122 | D | A | -1.5036 | |
| 123 | V | A | 0.0000 | |
| 124 | P | A | -0.2630 | |
| 125 | S | A | -0.1188 | |
| 126 | C | A | 0.0670 | |
| 127 | S | A | 0.0000 | |
| 128 | T | A | 0.0000 | |
| 129 | C | A | 0.0000 | |
| 130 | G | A | -0.1176 | |
| 131 | L | A | 0.1984 | |
| 132 | R | A | 0.0000 | |
| 133 | Q | A | -0.6122 | |
| 134 | Y | A | 0.4464 | |
| 135 | S | A | -0.1571 | |
| 136 | Q | A | -0.9202 | |
| 137 | P | A | -1.5104 | |
| 138 | Q | A | -2.0419 | |
| 139 | F | A | -1.0322 | |
| 140 | R | A | -1.1952 | |
| 141 | I | A | 0.9542 | |
| 142 | K | A | 0.4697 | |
| 143 | G | A | -0.1321 | |
| 144 | G | A | 0.2070 | |
| 145 | L | A | 1.6201 | |
| 146 | F | A | 0.8458 | |
| 147 | A | A | -0.2943 | |
| 148 | D | A | -1.3792 | |
| 149 | I | A | 0.0000 | |
| 150 | A | A | -0.4449 | |
| 151 | S | A | 0.0000 | |
| 152 | H | A | 0.0000 | |
| 153 | P | A | 0.0000 | |
| 154 | W | A | 0.0000 | |
| 155 | Q | A | 0.0000 | |
| 156 | A | A | 0.0000 | |
| 157 | A | A | 0.0000 | |
| 158 | I | A | 0.0000 | |
| 159 | F | A | 0.0000 | |
| 160 | A | A | 0.0000 | |
| 161 | K | A | -2.5054 | |
| 162 | H | A | -2.8174 | |
| 163 | R | A | -3.5455 | |
| 164 | R | A | -3.7109 | |
| 165 | S | A | -2.0817 | |
| 166 | P | A | -1.6215 | |
| 167 | G | A | -2.1972 | |
| 168 | E | A | -2.7189 | |
| 169 | R | A | -2.3189 | |
| 170 | F | A | -0.8227 | |
| 171 | L | A | -0.1172 | |
| 172 | C | A | 0.0000 | |
| 173 | G | A | 0.0000 | |
| 174 | G | A | 0.0000 | |
| 175 | I | A | 0.0000 | |
| 176 | L | A | 0.0000 | |
| 177 | I | A | 0.0000 | |
| 178 | S | A | -1.2121 | |
| 179 | S | A | -1.5965 | |
| 180 | C | A | 0.0000 | |
| 181 | W | A | 0.0000 | |
| 182 | I | A | 0.0000 | |
| 183 | L | A | 0.0000 | |
| 184 | S | A | 0.0000 | |
| 185 | A | A | 0.0000 | |
| 186 | A | A | 0.0000 | |
| 187 | H | A | -1.3990 | |
| 188 | C | A | -0.5002 | |
| 189 | F | A | 0.0000 | |
| 190 | Q | A | 0.0000 | |
| 191 | E | A | -2.6254 | |
| 192 | R | A | -2.6059 | |
| 193 | F | A | -1.6832 | |
| 194 | P | A | -1.4478 | |
| 195 | P | A | -1.7942 | |
| 196 | H | A | -1.9030 | |
| 197 | H | A | -2.0525 | |
| 198 | L | A | 0.0000 | |
| 199 | T | A | 0.0000 | |
| 200 | V | A | 0.0000 | |
| 201 | I | A | 0.0000 | |
| 202 | L | A | -0.3390 | |
| 203 | G | A | 0.0000 | |
| 204 | R | A | -0.5649 | |
| 205 | T | A | -0.2435 | |
| 206 | Y | A | 0.1721 | |
| 207 | R | A | -0.9218 | |
| 208 | R | A | -1.6046 | |
| 209 | V | A | -0.1965 | |
| 210 | P | A | -0.7343 | |
| 211 | G | A | -1.6111 | |
| 212 | E | A | -2.5432 | |
| 213 | E | A | -1.9320 | |
| 214 | E | A | -2.6981 | |
| 215 | Q | A | -2.5712 | |
| 216 | K | A | -2.3220 | |
| 217 | F | A | 0.0000 | |
| 218 | E | A | -3.2784 | |
| 219 | V | A | 0.0000 | |
| 220 | E | A | -2.4717 | |
| 221 | K | A | -1.8816 | |
| 222 | Y | A | -0.5013 | |
| 223 | I | A | -0.4043 | |
| 224 | V | A | -0.6664 | |
| 225 | H | A | -1.4538 | |
| 226 | K | A | -2.6185 | |
| 227 | E | A | -2.7364 | |
| 228 | F | A | -2.1378 | |
| 229 | D | A | -2.5332 | |
| 230 | D | A | -3.1114 | |
| 231 | D | A | -2.9938 | |
| 232 | T | A | -1.4579 | |
| 233 | Y | A | 0.0000 | |
| 234 | D | A | -1.9076 | |
| 235 | N | A | -1.7800 | |
| 236 | D | A | 0.0000 | |
| 237 | I | A | 0.0000 | |
| 238 | A | A | 0.0000 | |
| 239 | L | A | 0.0000 | |
| 240 | L | A | 0.0000 | |
| 241 | Q | A | 0.0000 | |
| 242 | L | A | 0.0000 | |
| 243 | K | A | -2.9655 | |
| 244 | S | A | -2.4119 | |
| 245 | D | A | -3.4740 | |
| 246 | S | A | 0.0000 | |
| 247 | S | A | 0.0000 | |
| 248 | R | A | -3.4768 | |
| 249 | C | A | -2.3746 | |
| 250 | A | A | -1.8571 | |
| 251 | Q | A | -2.8420 | |
| 252 | E | A | -3.1022 | |
| 253 | S | A | -2.0006 | |
| 254 | S | A | -1.2510 | |
| 255 | V | A | 0.2511 | |
| 256 | V | A | 0.0000 | |
| 257 | R | A | -1.3272 | |
| 258 | N | A | -1.0961 | mutated: RA258N |
| 259 | V | A | 0.0000 | |
| 260 | C | A | 0.2654 | |
| 261 | L | A | 0.1515 | |
| 262 | P | A | 0.0000 | |
| 263 | P | A | -0.6547 | |
| 264 | A | A | -1.1867 | |
| 265 | D | A | -2.1303 | |
| 266 | L | A | -1.0258 | |
| 267 | Q | A | -1.4628 | |
| 268 | L | A | 0.0000 | |
| 269 | P | A | -1.7002 | |
| 270 | D | A | -2.1978 | |
| 271 | W | A | -0.9832 | |
| 272 | T | A | -0.7721 | |
| 273 | E | A | -1.0849 | |
| 274 | C | A | 0.0000 | |
| 275 | E | A | 0.0000 | |
| 276 | L | A | 0.0000 | |
| 277 | S | A | 0.0000 | |
| 278 | G | A | 0.0000 | |
| 279 | Y | A | 0.0000 | |
| 280 | G | A | 0.0000 | |
| 281 | K | A | -0.4603 | |
| 282 | H | A | -0.4716 | |
| 283 | E | A | -1.5911 | |
| 284 | A | A | -0.0653 | |
| 285 | L | A | 1.1393 | |
| 286 | S | A | 0.4956 | |
| 287 | P | A | 0.9413 | |
| 288 | F | A | 1.8149 | |
| 289 | Y | A | 0.7024 | |
| 290 | S | A | -0.0135 | |
| 291 | E | A | 0.0000 | |
| 292 | R | A | -0.5617 | |
| 293 | L | A | 0.0000 | |
| 294 | K | A | 0.0295 | |
| 295 | E | A | 0.0000 | |
| 296 | A | A | 0.0000 | |
| 297 | H | A | -0.1698 | |
| 298 | V | A | 0.0000 | |
| 299 | R | A | -0.7895 | |
| 300 | L | A | 0.0000 | |
| 301 | Y | A | 0.0000 | |
| 302 | P | A | -1.0261 | |
| 303 | S | A | -1.2093 | |
| 304 | S | A | -1.2754 | |
| 305 | R | A | -2.4148 | |
| 306 | C | A | 0.0000 | |
| 307 | T | A | -0.4400 | |
| 308 | S | A | -0.3432 | |
| 309 | Q | A | 0.0000 | |
| 310 | H | A | 0.0000 | |
| 311 | L | A | 0.6089 | |
| 312 | L | A | 1.5021 | |
| 313 | N | A | -0.0667 | |
| 314 | R | A | -0.4675 | |
| 315 | T | A | 0.3732 | |
| 316 | V | A | 1.5683 | |
| 317 | T | A | 0.0843 | |
| 318 | D | A | -1.4678 | |
| 319 | N | A | 0.0000 | |
| 320 | M | A | 0.0000 | |
| 321 | L | A | -0.1324 | |
| 322 | C | A | 0.0000 | |
| 323 | A | A | 0.0000 | |
| 324 | G | A | 0.0000 | |
| 325 | D | A | -2.0228 | |
| 326 | T | A | -1.2324 | |
| 327 | R | A | 0.0000 | |
| 328 | S | A | -1.2737 | |
| 329 | G | A | -1.9037 | |
| 330 | G | A | -1.7877 | |
| 331 | P | A | -1.4906 | |
| 332 | Q | A | -1.8711 | |
| 333 | A | A | -0.9772 | |
| 334 | N | A | -0.5573 | |
| 335 | L | A | 0.6956 | |
| 336 | H | A | -0.0438 | |
| 337 | D | A | -0.2403 | |
| 338 | A | A | -0.1331 | |
| 339 | C | A | -0.6007 | |
| 340 | Q | A | 0.0000 | |
| 341 | G | A | 0.0000 | |
| 342 | D | A | 0.0000 | |
| 343 | S | A | -0.0909 | |
| 344 | G | A | 0.0000 | |
| 345 | G | A | 0.0000 | |
| 346 | P | A | 0.0000 | |
| 347 | L | A | 0.0000 | |
| 348 | V | A | 0.0000 | |
| 349 | C | A | 0.0000 | |
| 350 | L | A | -1.2863 | |
| 351 | N | A | 0.0000 | |
| 352 | D | A | -2.5475 | |
| 353 | G | A | -1.8168 | |
| 354 | R | A | -1.4798 | |
| 355 | M | A | 0.0000 | |
| 356 | T | A | 0.0000 | |
| 357 | L | A | 0.0000 | |
| 358 | V | A | -0.0019 | |
| 359 | G | A | 0.0000 | |
| 360 | I | A | 0.0000 | |
| 361 | I | A | 0.0000 | |
| 362 | S | A | -0.3162 | |
| 363 | W | A | -0.1946 | |
| 364 | G | A | -0.3570 | |
| 365 | L | A | 0.2893 | |
| 366 | G | A | -0.3958 | |
| 367 | C | A | 0.0000 | |
| 368 | G | A | -1.2301 | |
| 369 | Q | A | -1.7913 | |
| 370 | K | A | -1.2672 | |
| 371 | D | A | -1.7852 | |
| 372 | V | A | 0.0000 | |
| 373 | P | A | 0.0000 | |
| 374 | G | A | 0.0000 | |
| 375 | V | A | 0.0000 | |
| 376 | Y | A | 0.0000 | |
| 377 | T | A | 0.0000 | |
| 378 | K | A | -0.4014 | |
| 379 | V | A | 0.0000 | |
| 380 | T | A | 0.0000 | |
| 381 | N | A | 0.0000 | |
| 382 | Y | A | 0.0000 | |
| 383 | L | A | 0.0000 | |
| 384 | D | A | -1.4940 | |
| 385 | W | A | 0.0000 | |
| 386 | I | A | 0.0000 | |
| 387 | R | A | -2.8958 | |
| 388 | D | A | -3.1289 | |
| 389 | N | A | -2.0969 | |
| 390 | M | A | 0.0000 | |
| 391 | R | A | -2.9559 |
ALA alanine
ARG arginine
ASN asparagine
ASP aspartic acid
CYS cysteine
GLN glutamine
GLU glutamic acid
GLY glycine
HIS histidine
ILE isoleucine
LEU leucine
LYS lysine
MET methionine
PHE phenylalanine
PRO proline
SER serine
THR threonine
TRP tryptophan
TYR tyrosine
VAL valine