Project name: SH3_V141F

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	VA141F
Energy difference between WT (input) and mutated protein (by FoldX)	0.882379 kcal/mol Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 2.0032
• Average score: -0.9229
• Total score value: -52.6075

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5383
86	L	A	0.9203
87	F	A	1.0627
88	V	A	0.5633
89	A	A	0.0000
90	L	A	-0.1349
91	Y	A	-0.5878
92	D	A	-2.5604
93	Y	A	-1.9318
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.6898
105	K	A	-2.3682
106	G	A	-1.3665
107	E	A	-1.1258
108	K	A	-0.2316
109	F	A	0.0000
110	Q	A	-0.4424
111	I	A	-0.0242
112	L	A	0.1378
113	N	A	-0.8903
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0578
121	E	A	-1.1558
122	A	A	0.0000
123	R	A	-1.6441
124	S	A	0.0000
125	L	A	0.2468
126	T	A	-0.3475
127	T	A	-0.7582
128	G	A	-1.3376
129	E	A	-2.2285
130	T	A	-1.6870
131	G	A	-1.4991
132	Y	A	-0.8673
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1508
137	Y	A	-0.1398
138	V	A	0.0000
139	A	A	0.4746
140	P	A	0.8792
141	F	A	2.0032	mutated: VA141F