Project name: SH3_G128M

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	GA128M
Energy difference between WT (input) and mutated protein (by FoldX)	2.92793 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.795
• Average score: -0.8854
• Total score value: -50.4686

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5161
86	L	A	0.7676
87	F	A	0.8805
88	V	A	0.3863
89	A	A	0.0000
90	L	A	-0.1516
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7287
105	K	A	-2.4065
106	G	A	-1.4592
107	E	A	-1.3885
108	K	A	-0.7318
109	F	A	0.0000
110	Q	A	-0.3582
111	I	A	0.0044
112	L	A	0.1916
113	N	A	-0.8898
114	S	A	-1.1803
115	S	A	-1.5967
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0284
121	E	A	-1.1003
122	A	A	0.0000
123	R	A	-1.1272
124	S	A	0.0000
125	L	A	0.1530
126	T	A	-0.2544
127	T	A	-0.2028
128	M	A	-0.0238	mutated: GA128M
129	E	A	-1.5494
130	T	A	-1.2706
131	G	A	-1.3010
132	Y	A	-0.8315
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1504
137	Y	A	-0.1243
138	V	A	0.0000
139	A	A	0.4150
140	P	A	0.7744
141	V	A	1.7950