Project name: SH3_Y137N

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	YA137N
Energy difference between WT (input) and mutated protein (by FoldX)	2.03932 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7964
• Average score: -1.0053
• Total score value: -57.3016

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.8599
88	V	A	0.3453
89	A	A	0.0000
90	L	A	-0.3240
91	Y	A	-0.7072
92	D	A	-2.6300
93	Y	A	-2.0011
94	E	A	-2.6310
95	A	A	-2.6213
96	R	A	-2.9819
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.1965
103	F	A	0.0000
104	H	A	-2.7209
105	K	A	-2.4754
106	G	A	-1.4581
107	E	A	-1.3078
108	K	A	-0.6426
109	F	A	0.0000
110	Q	A	-0.5090
111	I	A	-0.0560
112	L	A	0.1431
113	N	A	-0.8875
114	S	A	-1.1774
115	S	A	-1.5946
116	E	A	-2.5574
117	G	A	-2.1307
118	D	A	-2.4481
119	W	A	-1.1734
120	W	A	-1.0607
121	E	A	-1.1557
122	A	A	0.0000
123	R	A	-1.7154
124	S	A	0.0000
125	L	A	0.0592
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6927
131	G	A	-1.4996
132	Y	A	-0.8738
133	I	A	0.0000
134	P	A	-0.7445
135	S	A	-1.1077
136	N	A	-1.4265
137	N	A	-0.6835	mutated: YA137N
138	V	A	0.0000
139	A	A	0.2983
140	P	A	0.7757
141	V	A	1.7964