Project name: SH3_F87E

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	FA87E
Energy difference between WT (input) and mutated protein (by FoldX)	5.45272 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7173
• Average score: -0.9852
• Total score value: -56.1568

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.4689
86	L	A	0.6895
87	E	A	0.0000	mutated: FA87E
88	V	A	0.3181
89	A	A	0.0000
90	L	A	-0.1785
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4857
107	E	A	-1.3428
108	K	A	-0.7106
109	F	A	0.0000
110	Q	A	-0.5419
111	I	A	-0.0529
112	L	A	0.1701
113	N	A	-0.8642
114	S	A	-1.1557
115	S	A	-1.5893
116	E	A	-2.5520
117	G	A	-2.1258
118	D	A	-2.4337
119	W	A	-1.0835
120	W	A	-1.0082
121	E	A	-1.1233
122	A	A	0.0000
123	R	A	-1.7018
124	S	A	0.0000
125	L	A	0.0353
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6881
131	G	A	-1.4888
132	Y	A	-0.8500
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9337
136	N	A	-1.1463
137	Y	A	-0.1509
138	V	A	0.0000
139	A	A	0.3367
140	P	A	0.6755
141	V	A	1.7173