Project name: SH3_S135I

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	SA135I
Energy difference between WT (input) and mutated protein (by FoldX)	3.99094 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7897
• Average score: -0.9322
• Total score value: -53.1373

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5106
86	L	A	0.7811
87	F	A	0.9601
88	V	A	0.4217
89	A	A	0.0000
90	L	A	-0.1534
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4604
107	E	A	-1.3108
108	K	A	-0.6500
109	F	A	0.0000
110	Q	A	-0.5230
111	I	A	-0.0252
112	L	A	0.1350
113	N	A	-0.8892
114	S	A	-1.0930
115	S	A	-1.5853
116	E	A	-2.5496
117	G	A	-2.0514
118	D	A	-2.2891
119	W	A	-0.9565
120	W	A	-0.9459
121	E	A	-1.1079
122	A	A	0.0000
123	R	A	-1.7219
124	S	A	0.0000
125	L	A	0.0571
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6941
131	G	A	-1.5060
132	Y	A	-0.8134
133	I	A	0.0000
134	P	A	0.0000
135	I	A	-0.4581	mutated: SA135I
136	N	A	-0.9279
137	Y	A	0.0250
138	V	A	0.0000
139	A	A	0.4878
140	P	A	0.7672
141	V	A	1.7897