Project name: SH3_E107F

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	EA107F
Energy difference between WT (input) and mutated protein (by FoldX)	-0.889383 kcal/mol Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1042
• Maximal score value: 1.7969
• Average score: -0.9124
• Total score value: -52.004

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	1.0249
88	V	A	0.6388
89	A	A	0.0000
90	L	A	-0.0520
91	Y	A	-0.4904
92	D	A	-2.4716
93	Y	A	-1.9352
94	E	A	-2.6499
95	A	A	-2.6304
96	R	A	-2.9870
97	T	A	-2.6622
98	E	A	-3.1042
99	D	A	-3.0446
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2149
103	F	A	0.0000
104	H	A	-2.5281
105	K	A	-2.1983
106	G	A	-1.1349
107	F	A	-0.6468	mutated: EA107F
108	K	A	-0.3220
109	F	A	0.0000
110	Q	A	-0.5091
111	I	A	-0.0568
112	L	A	0.1429
113	N	A	-0.8879
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0557
121	E	A	-1.1530
122	A	A	0.0000
123	R	A	-1.7086
124	S	A	0.0000
125	L	A	0.2005
126	T	A	-0.3060
127	T	A	-0.7336
128	G	A	-1.3454
129	E	A	-2.2208
130	T	A	-1.6838
131	G	A	-1.4965
132	Y	A	-0.8688
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1504
137	Y	A	-0.1243
138	V	A	0.0000
139	A	A	0.4163
140	P	A	0.7757
141	V	A	1.7969