Project name: SH3_G131W

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	GA131W
Energy difference between WT (input) and mutated protein (by FoldX)	6.40098 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.0187
• Maximal score value: 1.7964
• Average score: -0.881
• Total score value: -50.2191

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5137
86	L	A	0.7912
87	F	A	0.9206
88	V	A	0.4312
89	A	A	0.0000
90	L	A	-0.1501
91	Y	A	-0.5739
92	D	A	-2.5570
93	Y	A	-1.9281
94	E	A	-2.6409
95	A	A	-2.5662
96	R	A	-2.9836
97	T	A	-2.6596
98	E	A	-3.0187
99	D	A	-2.8939
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.0421
103	F	A	0.0000
104	H	A	-2.7240
105	K	A	-2.4038
106	G	A	-1.4548
107	E	A	-1.3010
108	K	A	-0.6368
109	F	A	0.0000
110	Q	A	-0.5102
111	I	A	-0.0057
112	L	A	0.2037
113	N	A	-0.8911
114	S	A	-1.1801
115	S	A	-1.5980
116	E	A	-2.5626
117	G	A	-2.1364
118	D	A	-2.4446
119	W	A	-1.0462
120	W	A	-0.9752
121	E	A	-0.9937
122	A	A	0.0000
123	R	A	-1.3405
124	S	A	0.0000
125	L	A	0.1835
126	T	A	-0.3517
127	T	A	-0.5824
128	G	A	-0.9477
129	E	A	-1.4162
130	T	A	-1.0829
131	W	A	-0.8475	mutated: GA131W
132	Y	A	-0.6066
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9199
136	N	A	-1.1508
137	Y	A	-0.1246
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7739
141	V	A	1.7964