Project name: SH3_Q110N

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	QA110N
Energy difference between WT (input) and mutated protein (by FoldX)	0.59957 kcal/mol Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7889
• Average score: -0.9761
• Total score value: -55.6356

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.4703
86	L	A	0.7250
87	F	A	0.8782
88	V	A	0.4199
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0435
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2060
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.2929
108	K	A	-0.6528
109	F	A	0.0000
110	N	A	-0.6671	mutated: QA110N
111	I	A	-0.2406
112	L	A	-0.0265
113	N	A	-0.9759
114	S	A	-1.2213
115	S	A	-1.6093
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.1036
121	E	A	-1.2193
122	A	A	0.0000
123	R	A	-1.7529
124	S	A	0.0000
125	L	A	0.1102
126	T	A	-0.4351
127	T	A	-0.7984
128	G	A	-1.3445
129	E	A	-2.2234
130	T	A	-1.7117
131	G	A	-1.5146
132	Y	A	-0.8847
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9289
136	N	A	-1.1504
137	Y	A	-0.1243
138	V	A	0.0000
139	A	A	0.4122
140	P	A	0.7579
141	V	A	1.7889