Project name: SH3_Y132A

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	YA132A
Energy difference between WT (input) and mutated protein (by FoldX)	1.40777 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.2025
• Maximal score value: 1.7964
• Average score: -1.0374
• Total score value: -59.1303

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5183
86	L	A	0.7945
87	F	A	0.9250
88	V	A	0.4313
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6543
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1300
99	D	A	-3.2025
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2343
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3078
108	K	A	-0.6420
109	F	A	0.0000
110	Q	A	-0.6159
111	I	A	-0.2910
112	L	A	-0.1386
113	N	A	-1.1765
114	S	A	-1.3555
115	S	A	-1.6692
116	E	A	-2.6448
117	G	A	-2.1783
118	D	A	-2.4951
119	W	A	-1.2275
120	W	A	-1.4723
121	E	A	-2.0530
122	A	A	0.0000
123	R	A	-1.9177
124	S	A	0.0000
125	L	A	0.0592
126	T	A	-0.4730
127	T	A	-0.8206
128	G	A	-1.3579
129	E	A	-2.2417
130	T	A	-1.9276
131	G	A	-1.8755
132	A	A	-1.3939	mutated: YA132A
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-1.0099
136	N	A	-1.1297
137	Y	A	-0.1097
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7757
141	V	A	1.7964