Project name: SH3_W120E

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	WA120E
Energy difference between WT (input) and mutated protein (by FoldX)	3.58642 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.8392
• Average score: -0.986
• Total score value: -56.2041

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5499
86	L	A	0.8576
87	F	A	1.0479
88	V	A	0.4925
89	A	A	0.0000
90	L	A	-0.1364
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4432
107	E	A	-1.2889
108	K	A	-0.6018
109	F	A	0.0000
110	Q	A	-0.5065
111	I	A	-0.1135
112	L	A	0.0649
113	N	A	-1.0271
114	S	A	-1.3796
115	S	A	-1.6893
116	E	A	-2.6482
117	G	A	-2.2226
118	D	A	-2.5627
119	W	A	-1.3003
120	E	A	-1.4387	mutated: WA120E
121	E	A	-1.3425
122	A	A	0.0000
123	R	A	-1.7494
124	S	A	0.0000
125	L	A	0.0721
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6908
131	G	A	-1.5573
132	Y	A	-0.9957
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-1.0389
136	N	A	-1.2007
137	Y	A	-0.1179
138	V	A	0.0000
139	A	A	0.4545
140	P	A	0.8298
141	V	A	1.8392