Project name: SH3_R123H

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	RA123H
Energy difference between WT (input) and mutated protein (by FoldX)	3.04525 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1026
• Maximal score value: 1.7964
• Average score: -0.8842
• Total score value: -50.4009

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.4584
86	L	A	0.7837
87	F	A	0.8554
88	V	A	0.3962
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5597
93	Y	A	-1.9312
94	E	A	-2.6448
95	A	A	-2.6254
96	R	A	-2.9850
97	T	A	-2.6607
98	E	A	-3.1026
99	D	A	-3.0511
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2028
103	F	A	0.0000
104	H	A	-2.7280
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3042
108	K	A	-0.5646
109	F	A	0.0000
110	Q	A	-0.3717
111	I	A	0.3094
112	L	A	0.8546
113	N	A	-0.6349
114	S	A	-1.0498
115	S	A	-1.5277
116	E	A	-2.5662
117	G	A	-2.1386
118	D	A	-2.4493
119	W	A	-1.1127
120	W	A	-0.8854
121	E	A	-0.8519
122	A	A	0.0000
123	H	A	-0.8420	mutated: RA123H
124	S	A	0.0000
125	L	A	0.1967
126	T	A	-0.3748
127	T	A	-0.7417
128	G	A	-1.2545
129	E	A	-2.0040
130	T	A	-1.3017
131	G	A	-1.2585
132	Y	A	-0.6983
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9256
136	N	A	-1.1504
137	Y	A	-0.1243
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7465
141	V	A	1.7964