Project name: SH3_V88G

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	VA88G
Energy difference between WT (input) and mutated protein (by FoldX)	2.6163 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.6553
• Average score: -0.9966
• Total score value: -56.8076

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.4698
86	L	A	0.6762
87	F	A	0.6896
88	G	A	-0.0338	mutated: VA88G
89	A	A	0.0000
90	L	A	-0.2096
91	Y	A	-0.6015
92	D	A	-2.5471
93	Y	A	-1.9238
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7782
105	K	A	-2.4794
106	G	A	-1.5992
107	E	A	-1.4629
108	K	A	-0.8816
109	F	A	0.0000
110	Q	A	-0.5734
111	I	A	-0.0638
112	L	A	0.1430
113	N	A	-0.8879
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0557
121	E	A	-1.1529
122	A	A	0.0000
123	R	A	-1.7141
124	S	A	0.0000
125	L	A	0.0308
126	T	A	-0.4656
127	T	A	-0.8148
128	G	A	-1.3446
129	E	A	-2.2334
130	T	A	-1.6879
131	G	A	-1.4991
132	Y	A	-0.8673
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9200
136	N	A	-1.1409
137	Y	A	-0.1748
138	V	A	0.0000
139	A	A	0.2775
140	P	A	0.6494
141	V	A	1.6553