Project name: 3TL2 mesophile malate-dehydrogenase

Chain sequence(s)	A: MTIKRKKVSVIGAGFTTGATTAFLLAQKELADVVLVDIPQLENPTKGKALDMLEASPVQQGFDANIIGTSDYADTADSDVVVITAGIARKPGMMSRDDLVATNSKIMKSITRDIAKHSPNAIIVVLTNPVDAMTYSVFKEAGFPKERVIIGQSGVLDTARFRTFIAQELNLSVKDITGFVLGGGHGDDMVPLVVRYSYAGGIPLETLIPKERLEAIVERTRKGGGEIVGLLGNGSAYYAPAASLVEMTEAILKDQRRVLPAIAYLEGEYGYSDLYLGVPVILGGNGIEKIIELELLADEKEALDRSVESVRNVMKVLV
Distance of aggregation	10 Å
Dynamic mode	No

• Minimal score value: -3.9256
• Maximal score value: 1.8243
• Average score: -0.7616
• Total score value: -237.6229

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
1	M	A	1.3139
2	T	A	0.7268
3	I	A	0.9569
4	K	A	-1.7318
5	R	A	-3.2345
6	K	A	-2.8123
7	K	A	-2.3381
8	V	A	0.0000
9	S	A	0.0000
10	V	A	0.0000
11	I	A	0.0000
12	G	A	0.0000
13	A	A	0.0000
14	G	A	0.3441
15	F	A	1.8243
16	T	A	1.2364
17	G	A	0.0000
18	A	A	0.5615
19	T	A	0.9942
20	T	A	0.0000
21	A	A	0.0000
22	F	A	-0.0781
23	L	A	-0.0577
24	L	A	0.0000
25	A	A	0.0000
26	Q	A	-1.8058
27	K	A	-2.4403
28	E	A	-3.4110
29	L	A	0.0000
30	A	A	0.0000
31	D	A	-2.1546
32	V	A	0.0000
33	V	A	0.0000
34	L	A	0.0000
35	V	A	0.0000
36	D	A	-0.4419
37	I	A	0.5050
38	P	A	-0.8079
39	Q	A	-1.0442
40	L	A	-0.5212
41	E	A	-1.8668
42	N	A	-2.3271
43	P	A	-1.5267
44	T	A	0.0000
45	K	A	-1.6584
46	G	A	-1.5818
47	K	A	-1.4263
48	A	A	0.0000
49	L	A	-0.6035
50	D	A	-2.1308
51	M	A	0.0000
52	L	A	-0.8722
53	E	A	-1.8550
54	A	A	-0.8568
55	S	A	-1.3210
56	P	A	-0.5825
57	V	A	0.6637
58	Q	A	-1.1013
59	G	A	-1.2356
60	F	A	0.0000
61	D	A	-3.0267
62	A	A	-2.3983
63	N	A	-1.7540
64	I	A	0.0000
65	I	A	0.4572
66	G	A	0.0000
67	T	A	0.0000
68	S	A	-1.2838
69	D	A	-2.0050
70	Y	A	-1.2738
71	A	A	-1.4428
72	D	A	-1.4175
73	T	A	0.0000
74	A	A	-1.9055
75	D	A	-2.8929
76	S	A	0.0000
77	D	A	-2.3842
78	V	A	0.0000
79	V	A	0.0000
80	V	A	0.0000
81	I	A	0.0000
82	T	A	0.2732
83	A	A	0.3000
84	G	A	0.1639
85	I	A	0.8257
86	A	A	-0.1660
87	R	A	-1.3621
88	K	A	-2.0489
89	P	A	-1.5739
90	G	A	-1.0533
91	M	A	-1.4275
92	S	A	-1.8767
93	R	A	-2.5223
94	D	A	-2.5947
95	D	A	-2.5381
96	L	A	0.0000
97	V	A	0.0000
98	A	A	-0.8528
99	T	A	-0.8758
100	N	A	0.0000
101	S	A	-0.4163
102	K	A	-1.2518
103	I	A	-0.0516
104	M	A	0.0000
105	K	A	-1.8971
106	S	A	-1.3993
107	I	A	-1.1490
108	T	A	0.0000
109	R	A	-3.0286
110	D	A	-2.3593
111	I	A	0.0000
112	A	A	-2.3695
113	K	A	-2.9485
114	H	A	-2.2838
115	S	A	0.0000
116	P	A	-2.0222
117	N	A	-2.2985
118	A	A	0.0000
119	I	A	0.0000
120	I	A	0.0000
121	V	A	0.0000
122	V	A	0.0000
123	L	A	0.1051
124	T	A	0.0000
125	N	A	-0.4825
126	P	A	0.0000
127	V	A	0.0000
128	D	A	0.0000
129	A	A	0.0000
130	M	A	0.0000
131	T	A	0.0000
132	Y	A	-0.5167
133	S	A	-0.6288
134	V	A	0.0000
135	F	A	-1.0436
136	K	A	-2.1052
137	E	A	-2.1396
138	A	A	0.0000
139	G	A	-1.5824
140	F	A	0.0000
141	P	A	-1.0962
142	K	A	-1.2507
143	E	A	-1.7257
144	R	A	-1.5000
145	V	A	0.0000
146	I	A	0.0000
147	G	A	0.0000
148	Q	A	0.0000
149	S	A	0.0000
150	G	A	0.0000
151	V	A	0.0000
152	L	A	0.0000
153	D	A	0.0000
154	T	A	0.0000
155	A	A	0.0117
156	R	A	0.0000
157	F	A	0.0000
158	R	A	-0.9031
159	T	A	-0.4892
160	F	A	-0.7124
161	I	A	0.0000
162	A	A	0.0000
163	Q	A	-1.6471
164	E	A	-1.5701
165	L	A	-0.6395
166	N	A	-1.2769
167	L	A	-0.5906
168	S	A	-0.9265
169	V	A	-0.7953
170	K	A	-2.1610
171	D	A	-2.2793
172	I	A	0.0000
173	T	A	-0.4388
174	G	A	0.0000
175	F	A	0.4135
176	V	A	0.0000
177	L	A	0.0000
178	G	A	0.0000
179	G	A	0.0000
180	H	A	-1.0536
181	G	A	-2.1237
182	D	A	-2.6974
183	D	A	-1.8383
184	M	A	0.0000
185	V	A	0.0000
186	P	A	0.0310
187	L	A	0.0000
188	V	A	-0.2782
189	R	A	-0.4804
190	Y	A	0.2255
191	S	A	0.0000
192	Y	A	0.1506
193	A	A	0.0000
194	G	A	-0.8153
195	G	A	-0.0772
196	I	A	1.0302
197	P	A	0.2353
198	L	A	0.0000
199	E	A	-1.7596
200	T	A	-0.5404
201	L	A	0.3230
202	I	A	0.0000
203	P	A	-2.4578
204	K	A	-3.8347
205	E	A	-3.8953
206	R	A	-3.4306
207	L	A	0.0000
208	E	A	-3.9256
209	A	A	-2.9338
210	I	A	0.0000
211	V	A	0.0000
212	E	A	-3.7526
213	R	A	-3.0446
214	T	A	0.0000
215	R	A	-2.7840
216	K	A	-3.1520
217	G	A	0.0000
218	G	A	-1.2189
219	G	A	-1.2467
220	E	A	-0.6540
221	I	A	0.4494
222	V	A	0.0000
223	G	A	0.0173
224	L	A	1.5760
225	L	A	1.1175
226	G	A	-0.2084
227	N	A	-1.1282
228	G	A	-0.5863
229	S	A	-0.0266
230	A	A	0.8636
231	Y	A	1.3282
232	Y	A	1.7644
233	A	A	0.7443
234	P	A	0.0000
235	A	A	0.0000
236	A	A	0.3234
237	S	A	0.0000
238	L	A	0.0000
239	V	A	0.0000
240	E	A	-0.7491
241	M	A	0.0000
242	T	A	0.0000
243	E	A	-1.3740
244	A	A	0.0000
245	I	A	0.0000
246	L	A	-1.5871
247	K	A	-2.2722
248	D	A	-2.8907
249	Q	A	-2.8205
250	R	A	-2.9788
251	R	A	-1.2018
252	V	A	0.9320
253	L	A	0.7108
254	P	A	0.5038
255	A	A	0.0000
256	I	A	0.0000
257	A	A	0.0000
258	Y	A	0.0000
259	L	A	0.0000
260	E	A	-2.7544
261	G	A	-1.9075
262	E	A	-1.5220
263	Y	A	0.0000
264	G	A	-1.3261
265	Y	A	0.0000
266	S	A	-2.0613
267	D	A	-2.4903
268	L	A	0.0000
269	Y	A	0.0000
270	L	A	0.0000
271	G	A	0.0000
272	V	A	0.0000
273	P	A	0.0000
274	V	A	0.0000
275	I	A	-0.5512
276	L	A	0.0000
277	G	A	0.0000
278	G	A	-2.2371
279	N	A	-2.2128
280	G	A	0.0000
281	I	A	0.0000
282	E	A	-1.9587
283	K	A	-1.7544
284	I	A	0.0000
285	I	A	-0.5392
286	E	A	-2.1333
287	L	A	-1.2448
288	E	A	-1.8076
289	L	A	-0.9473
290	L	A	-0.1117
291	A	A	-1.0776
292	D	A	-2.2746
293	E	A	-1.9007
294	K	A	-2.3372
295	E	A	-3.0932
296	A	A	-2.8298
297	L	A	0.0000
298	D	A	-3.3948
299	R	A	-3.8258
300	S	A	0.0000
301	V	A	0.0000
302	E	A	-3.2198
303	S	A	-2.4027
304	V	A	0.0000
305	R	A	-2.5052
306	N	A	-2.1209
307	V	A	0.0000
308	M	A	-1.1134
309	K	A	-1.4544
310	V	A	-0.3941
311	L	A	0.5932
312	V	A	1.5813