Project name: SH3_T127L

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	TA127L
Energy difference between WT (input) and mutated protein (by FoldX)	-0.0554598 kcal/mol Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.0714
• Maximal score value: 1.7964
• Average score: -0.8929
• Total score value: -50.8949

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9298
88	V	A	0.4435
89	A	A	0.0000
90	L	A	-0.1453
91	Y	A	-0.5698
92	D	A	-2.5547
93	Y	A	-1.9067
94	E	A	-2.6152
95	A	A	-2.5751
96	R	A	-2.9614
97	T	A	-2.6361
98	E	A	-3.0714
99	D	A	-3.0066
100	D	A	0.0000
101	L	A	-1.8428
102	S	A	-2.1324
103	F	A	0.0000
104	H	A	-2.7164
105	K	A	-2.3937
106	G	A	-1.4392
107	E	A	-1.0828
108	K	A	-0.4583
109	F	A	0.0000
110	Q	A	-0.5091
111	I	A	-0.0568
112	L	A	0.1429
113	N	A	-0.8883
114	S	A	-1.1799
115	S	A	-1.5966
116	E	A	-2.5603
117	G	A	-2.1347
118	D	A	-2.4456
119	W	A	-1.1053
120	W	A	-1.0569
121	E	A	-1.1340
122	A	A	0.0000
123	R	A	-1.4363
124	S	A	0.0000
125	L	A	0.5358
126	T	A	0.3173
127	L	A	0.8024	mutated: TA127L
128	G	A	-0.5644
129	E	A	-1.6883
130	T	A	-1.4394
131	G	A	-1.4433
132	Y	A	-0.8211
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9214
136	N	A	-1.1508
137	Y	A	-0.1246
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7757
141	V	A	1.7964