Project name: SH3_V87N

Chain sequence(s)	A: GSHMTFVALYDYESRTETDLSFKKGERLQIVNNTEGDWWLAHSLTTGQTGYIPSNYVAPS
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	VA87N
Energy difference between WT (input) and mutated protein (by FoldX)	1.0205 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.579
• Maximal score value: 1.2498
• Average score: -0.9569
• Total score value: -57.4138

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
81	G	A	-0.4510
82	S	A	-0.6826
83	H	A	-0.7912
84	M	A	0.2661
85	T	A	0.0000
86	F	A	-0.5877
87	N	A	-1.6079	mutated: VA87N
88	A	A	0.0000
89	L	A	-0.5169
90	Y	A	-0.8314
91	D	A	-2.8405
92	Y	A	-2.0987
93	E	A	-2.8815
94	S	A	0.0000
95	R	A	-2.7837
96	T	A	-2.1540
97	E	A	-2.3526
98	T	A	-1.2414
99	D	A	-1.3229
100	L	A	0.0000
101	S	A	-1.9034
102	F	A	0.0000
103	K	A	-3.5790
104	K	A	-3.0280
105	G	A	-2.2480
106	E	A	0.0000
107	R	A	-2.4008
108	L	A	0.0000
109	Q	A	-0.3754
110	I	A	0.4404
111	V	A	1.2498
112	N	A	-0.4200
113	N	A	-1.8142
114	T	A	-1.7328
115	E	A	-2.9363
116	G	A	-2.6085
117	D	A	-2.6844
118	W	A	-1.3424
119	W	A	-0.6978
120	L	A	0.4047
121	A	A	0.0000
122	H	A	-0.3786
123	S	A	0.0000
124	L	A	-0.2680
125	T	A	-0.7689
126	T	A	-0.8729
127	G	A	-0.8169
128	Q	A	-1.4119
129	T	A	-0.4950
130	G	A	0.0000
131	Y	A	0.2197
132	I	A	0.0000
133	P	A	0.0000
134	S	A	-1.2853
135	N	A	-1.2391
136	Y	A	-0.3337
137	V	A	0.0000
138	A	A	-0.3227
139	P	A	-0.4166
140	S	A	-0.4699