Project name: 20190218h8C12woDyn [mutate: AH98L, SH149I, EH178A] [mutate: VH120K, LH128K, LH132K]

Chain sequence(s)	H: QVQLQESGPGLVKPSETLSLTCTVSGFSLNSFGWSWIRQPPGKGLEWIGYIWAGKNTNYNPSLKSRVTISVDTSKNQFSLKLSSVTAADTAVYYCARLGYGNSLDYWGQGTTLTVSSDIVMTQSPDSLAVSLGERATINCKSSQSLLNIGNQRNYLTWYQQKPGQPPKLLIYWASTRASGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQNNYYFPLTFGTGTKLEIK
Distance of aggregation	5 Å
Dynamic mode	No
Mutated residues	VH120K, LH128K, LH132K
Energy difference between WT (input) and mutated protein (by FoldX)	1.14467 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -2.2162
• Maximal score value: 1.7522
• Average score: -0.2857
• Total score value: -65.708

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
1	Q	H	-1.1983
2	V	H	0.0000
3	Q	H	-1.1983
4	L	H	0.0000
5	Q	H	-0.7656
6	E	H	0.0000
7	S	H	-0.2502
8	G	H	-0.5234
9	P	H	-0.3698
10	G	H	-0.1551
11	L	H	1.2653
12	V	H	0.0000
13	K	H	-1.6845
14	P	H	-0.4958
15	S	H	-0.4167
16	E	H	-0.8205
17	T	H	-0.2116
18	L	H	0.0000
19	S	H	-0.3001
20	L	H	0.0000
21	T	H	-0.0232
22	C	H	0.0000
23	T	H	-0.0473
24	V	H	0.0000
25	S	H	-0.2830
26	G	H	-0.4511
27	F	H	0.1782
28	S	H	-0.0304
29	L	H	0.0000
30	N	H	-1.0657
31	S	H	-0.2322
32	F	H	0.7120
33	G	H	0.0000
34	W	H	0.0000
35	S	H	0.0000
36	W	H	0.0000
37	I	H	0.0000
38	R	H	0.0000
39	Q	H	-0.1305
40	P	H	-0.0999
41	P	H	-0.3466
42	G	H	-0.8280
43	K	H	-1.7985
44	G	H	-0.3895
45	L	H	0.0000
46	E	H	-0.5636
47	W	H	0.0000
48	I	H	0.0000
49	G	H	0.0000
50	Y	H	0.1452
51	I	H	0.0000
52	W	H	0.5276
53	A	H	-0.0571
54	G	H	-0.7676
55	K	H	-2.0205
56	N	H	-1.5913
57	T	H	-0.3462
58	N	H	-0.4461
59	Y	H	0.1645
60	N	H	-0.1076
61	P	H	-0.2549
62	S	H	-0.2482
63	L	H	0.0000
64	K	H	-1.7391
65	S	H	-0.5936
66	R	H	-0.4175
67	V	H	0.0000
68	T	H	-0.0263
69	I	H	0.0000
70	S	H	-0.1118
71	V	H	0.0902
72	D	H	-0.5015
73	T	H	-0.2196
74	S	H	-0.5348
75	K	H	-1.7866
76	N	H	-0.6165
77	Q	H	-0.2671
78	F	H	0.0000
79	S	H	-0.0591
80	L	H	0.0000
81	K	H	-0.9470
82	L	H	0.0000
83	S	H	-0.1451
84	S	H	-0.2586
85	V	H	0.0000
86	T	H	-0.0421
87	A	H	0.0593
88	A	H	0.0691
89	D	H	0.0000
90	T	H	-0.0182
91	A	H	0.0000
92	V	H	0.4243
93	Y	H	0.0000
94	Y	H	0.0000
95	C	H	0.0000
96	A	H	0.0000
97	R	H	0.0000
98	L	H	0.0000
99	G	H	0.1704
100	Y	H	1.2189
101	G	H	-0.2291
102	N	H	0.0000
103	S	H	-0.0254
104	L	H	0.0000
105	D	H	-1.4531
106	Y	H	0.1070
107	W	H	0.2293
108	G	H	0.0000
109	Q	H	-1.1983
110	G	H	0.0000
111	T	H	-0.0237
112	T	H	-0.0118
113	L	H	0.0000
114	T	H	-0.0268
115	V	H	0.0000
116	S	H	-0.1472
117	S	H	-0.2329
118	D	H	-1.7520
119	I	H	-0.4364
120	K	H	-1.6628	mutated: VH120K
121	M	H	0.0000
122	T	H	-0.0345
123	Q	H	0.0000
124	S	H	-0.2241
125	P	H	-0.4770
126	D	H	-1.8460
127	S	H	-0.7498
128	K	H	-0.4714	mutated: LH128K
129	A	H	-0.4227
130	V	H	0.0000
131	S	H	-0.6534
132	K	H	-1.8106	mutated: LH132K
133	G	H	-1.1160
134	E	H	-2.2162
135	R	H	-2.1880
136	A	H	0.0000
137	T	H	-0.0292
138	I	H	0.0000
139	N	H	-0.9045
140	C	H	0.0000
141	K	H	-1.3820
142	S	H	0.0000
143	S	H	-0.3770
144	Q	H	-1.2512
145	S	H	-0.3621
146	L	H	0.0000
147	L	H	0.2191
148	N	H	0.2258
149	I	H	1.7522
150	G	H	-0.3161
151	N	H	-1.4588
152	Q	H	-1.3527
153	R	H	-1.2030
154	N	H	-0.2282
155	Y	H	0.1857
156	L	H	0.0000
157	T	H	0.0000
158	W	H	0.0000
159	Y	H	0.0000
160	Q	H	0.0000
161	Q	H	0.0000
162	K	H	-0.7657
163	P	H	-0.4708
164	G	H	-0.7274
165	Q	H	-1.2652
166	P	H	-0.2794
167	P	H	0.0000
168	K	H	-1.4560
169	L	H	-0.0340
170	L	H	0.0000
171	I	H	0.0000
172	Y	H	0.2767
173	W	H	0.4308
174	A	H	0.0000
175	S	H	-0.2187
176	T	H	-0.2009
177	R	H	-0.7520
178	A	H	-0.1682
179	S	H	-0.2969
180	G	H	-0.5053
181	V	H	0.0000
182	P	H	-0.4217
183	D	H	-1.8607
184	R	H	-0.6331
185	F	H	0.0000
186	S	H	-0.1004
187	G	H	-0.1328
188	S	H	-0.2633
189	G	H	-0.3037
190	S	H	-0.2690
191	G	H	-0.1379
192	T	H	-0.3387
193	D	H	-1.7443
194	F	H	0.0000
195	T	H	-0.1333
196	L	H	0.0000
197	T	H	-0.0224
198	I	H	0.0000
199	S	H	-0.3809
200	S	H	-0.5526
201	L	H	0.0000
202	Q	H	-0.5072
203	A	H	-0.3695
204	E	H	-1.8092
205	D	H	0.0000
206	V	H	0.4870
207	A	H	0.0000
208	V	H	0.0508
209	Y	H	0.0000
210	Y	H	0.0000
211	C	H	0.0000
212	Q	H	0.0000
213	N	H	0.0000
214	N	H	-0.1068
215	Y	H	0.4330
216	Y	H	1.5691
217	F	H	1.3851
218	P	H	0.2635
219	L	H	0.0000
220	T	H	0.0235
221	F	H	0.2009
222	G	H	0.0000
223	T	H	-0.0777
224	G	H	0.0000
225	T	H	0.0000
226	K	H	-1.0746
227	L	H	0.0000
228	E	H	-1.5457
229	I	H	0.0239
230	K	H	-1.5930