Project name: SH3_Q110T

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	QA110T
Energy difference between WT (input) and mutated protein (by FoldX)	-0.0706795 kcal/mol Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7988
• Average score: -0.9287
• Total score value: -52.9376

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.6481
86	L	A	0.9893
87	F	A	1.0775
88	V	A	0.5134
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3114
108	K	A	-0.5630
109	F	A	0.0000
110	T	A	0.0559	mutated: QA110T
111	I	A	0.2524
112	L	A	0.4226
113	N	A	-0.7241
114	S	A	-1.1393
115	S	A	-1.5724
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-0.9918
121	E	A	-1.0759
122	A	A	0.0000
123	R	A	-1.8095
124	S	A	0.0000
125	L	A	0.0934
126	T	A	-0.5287
127	T	A	-0.8791
128	G	A	-1.3953
129	E	A	-2.3490
130	T	A	-1.7434
131	G	A	-1.5545
132	Y	A	-0.8937
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9230
136	N	A	-1.1504
137	Y	A	-0.1243
138	V	A	0.0000
139	A	A	0.4170
140	P	A	0.8426
141	V	A	1.7988