Project name: SH3_Y132Q

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	YA132Q
Energy difference between WT (input) and mutated protein (by FoldX)	1.16232 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.291
• Maximal score value: 1.7964
• Average score: -1.0599
• Total score value: -60.412

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5183
86	L	A	0.7945
87	F	A	0.9250
88	V	A	0.4313
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.7114
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1834
99	D	A	-3.2910
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2909
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3078
108	K	A	-0.6420
109	F	A	0.0000
110	Q	A	-0.5813
111	I	A	-0.2689
112	L	A	-0.0995
113	N	A	-1.1451
114	S	A	-1.3714
115	S	A	-1.6566
116	E	A	-2.6386
117	G	A	-2.2008
118	D	A	-2.6008
119	W	A	-1.4723
120	W	A	-1.5763
121	E	A	-2.0186
122	A	A	0.0000
123	R	A	-1.9276
124	S	A	0.0000
125	L	A	0.0592
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3579
129	E	A	-2.2416
130	T	A	-1.9632
131	G	A	-2.0303
132	Q	A	-1.7760	mutated: YA132Q
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-1.0959
136	N	A	-1.1562
137	Y	A	-0.1284
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7757
141	V	A	1.7964