Project name: SH3_S102V

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	SA102V
Energy difference between WT (input) and mutated protein (by FoldX)	1.61412 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -2.9196
• Maximal score value: 1.7964
• Average score: -0.8612
• Total score value: -49.0878

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9242
88	V	A	0.4320
89	A	A	0.0000
90	L	A	-0.1519
91	Y	A	-0.5768
92	D	A	-2.2831
93	Y	A	-1.4355
94	E	A	-2.0117
95	A	A	-2.0101
96	R	A	-2.7452
97	T	A	-2.4796
98	E	A	-2.9196
99	D	A	-2.8783
100	D	A	0.0000
101	L	A	0.0000
102	V	A	-0.6487	mutated: SA102V
103	F	A	0.0000
104	H	A	-2.4167
105	K	A	-2.4068
106	G	A	-1.4570
107	E	A	-1.3055
108	K	A	-0.6415
109	F	A	0.0000
110	Q	A	-0.5091
111	I	A	-0.0568
112	L	A	0.1429
113	N	A	-0.8883
114	S	A	-1.1799
115	S	A	-1.5966
116	E	A	-2.5603
117	G	A	-2.1347
118	D	A	-2.4456
119	W	A	-1.1053
120	W	A	-1.0569
121	E	A	-1.1536
122	A	A	0.0000
123	R	A	-1.6947
124	S	A	0.0000
125	L	A	0.0701
126	T	A	-0.4619
127	T	A	-0.7993
128	G	A	-1.3255
129	E	A	-1.9906
130	T	A	-1.4996
131	G	A	-1.2636
132	Y	A	-0.6667
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9214
136	N	A	-1.1508
137	Y	A	-0.1262
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7757
141	V	A	1.7964