| Chain sequence(s) |
A: GSPVDICTAKPRDIPMNPMCIYRSPEKKATEDEGSEQKIPEATNRRVWELSKANSRFATTFYQHLADSKNDNDNIFLSPLSISTAFAMTKLGACNDTLQQLMEVFKFDTISEKTSDQIHFFFAKLNCRLYRKANKSSKLVSANRLFGDKSLTFNETYQDISELVYGAKLQPLDFKENAEQSRAAINKWVSNKTEGRITDVIPSEAINELTVLVLVNTIYFKGLWKSKFSPENTRKELFYKADGESCSASMMYQEGKFRYRRVAEGTQVLELPFKGDDITMVLILPKPEKSLAKVEKELTPEVLQEWLDELEEMMLVVHMPRFRIEDGFSLKEQLQDMGLVDLFSPEKSKLPGIVAEGRDDLYVSDAFHKAFLEVNEEGSEAAASTAVVIAGRSLNPNRVTFKANRPFLVFIREVPLNTIIFMGRVANPCVK |
| Distance of aggregation | 10 Å |
| Dynamic mode | No |
| Mutated residues | CA247A, CA430A |
| Energy difference between WT (input) and mutated protein (by FoldX) | 5.3489 kcal/mol
CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive. |
The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.
| residue index | residue name | chain | Aggrescan3D score | mutation |
|---|---|---|---|---|
| residue index | residue name | chain | Aggrescan3D score | |
| 2 | G | A | -0.7171 | |
| 3 | S | A | -0.5859 | |
| 4 | P | A | -0.3784 | |
| 5 | V | A | -0.3938 | |
| 6 | D | A | -1.5049 | |
| 7 | I | A | -0.9781 | |
| 8 | C | A | -1.1598 | |
| 9 | T | A | -0.7791 | |
| 10 | A | A | -1.8312 | |
| 11 | K | A | -3.0744 | |
| 12 | P | A | -2.4677 | |
| 13 | R | A | -3.3546 | |
| 14 | D | A | -3.0550 | |
| 15 | I | A | -1.6060 | |
| 16 | P | A | -1.2234 | |
| 17 | M | A | -0.7616 | |
| 18 | N | A | -0.7781 | |
| 19 | P | A | 0.2744 | |
| 20 | M | A | 1.0393 | |
| 21 | C | A | 0.9250 | |
| 22 | I | A | 1.2916 | |
| 23 | Y | A | -0.3580 | |
| 24 | R | A | -1.8978 | |
| 25 | S | A | -2.0666 | |
| 26 | P | A | -2.5554 | |
| 27 | E | A | -3.5072 | |
| 28 | K | A | -3.3684 | |
| 29 | K | A | -2.7123 | |
| 30 | A | A | -2.0471 | |
| 31 | T | A | -2.1100 | |
| 32 | E | A | -3.0437 | |
| 33 | D | A | -3.3160 | |
| 34 | E | A | -3.5785 | |
| 35 | G | A | -2.8350 | |
| 36 | S | A | -2.9060 | |
| 37 | E | A | -3.4046 | |
| 38 | Q | A | -2.9054 | |
| 39 | K | A | -3.1172 | |
| 40 | I | A | -2.1274 | |
| 41 | P | A | -1.7156 | |
| 42 | E | A | -2.2551 | |
| 43 | A | A | -1.1253 | |
| 44 | T | A | -1.2958 | |
| 45 | N | A | -1.6656 | |
| 46 | R | A | -2.1588 | |
| 47 | R | A | -1.9619 | |
| 48 | V | A | 0.0000 | |
| 49 | W | A | -1.0518 | |
| 50 | E | A | -1.0234 | |
| 51 | L | A | 0.0000 | |
| 52 | S | A | 0.0000 | |
| 53 | K | A | -1.1373 | |
| 54 | A | A | 0.0000 | |
| 55 | N | A | 0.0000 | |
| 56 | S | A | -0.7854 | |
| 57 | R | A | -0.8634 | |
| 58 | F | A | 0.0000 | |
| 59 | A | A | 0.0000 | |
| 60 | T | A | 0.0000 | |
| 61 | T | A | -0.6105 | |
| 62 | F | A | 0.0000 | |
| 63 | Y | A | 0.0000 | |
| 64 | Q | A | -1.7767 | |
| 65 | H | A | -1.9993 | |
| 66 | L | A | 0.0000 | |
| 67 | A | A | -2.3815 | |
| 68 | D | A | -3.0776 | |
| 69 | S | A | -2.0962 | |
| 70 | K | A | -2.7687 | |
| 71 | N | A | -3.1612 | |
| 72 | D | A | -3.3226 | |
| 73 | N | A | -2.6454 | |
| 74 | D | A | -2.3810 | |
| 75 | N | A | -1.9732 | |
| 76 | I | A | 0.0000 | |
| 77 | F | A | 0.0000 | |
| 78 | L | A | 0.0000 | |
| 79 | S | A | 0.0000 | |
| 80 | P | A | 0.0000 | |
| 81 | L | A | 0.0000 | |
| 82 | S | A | 0.0000 | |
| 83 | I | A | 0.0000 | |
| 84 | S | A | 0.0000 | |
| 85 | T | A | 0.0000 | |
| 86 | A | A | 0.0000 | |
| 87 | F | A | 0.0000 | |
| 88 | A | A | 0.0000 | |
| 89 | M | A | 0.0000 | |
| 90 | T | A | 0.0000 | |
| 91 | K | A | 0.0000 | |
| 92 | L | A | 0.0000 | |
| 93 | G | A | 0.0000 | |
| 94 | A | A | 0.0000 | |
| 95 | C | A | -0.9050 | |
| 96 | N | A | -2.4042 | |
| 97 | D | A | -3.0224 | |
| 98 | T | A | 0.0000 | |
| 99 | L | A | 0.0000 | |
| 100 | Q | A | -2.3244 | |
| 101 | Q | A | -1.9566 | |
| 102 | L | A | 0.0000 | |
| 103 | M | A | 0.0000 | |
| 104 | E | A | -2.5865 | |
| 105 | V | A | 0.0000 | |
| 106 | F | A | 0.0000 | |
| 107 | K | A | -1.5030 | |
| 108 | F | A | 0.0000 | |
| 109 | D | A | -2.2611 | |
| 110 | T | A | -1.2010 | |
| 111 | I | A | 0.0000 | |
| 112 | S | A | 0.0000 | |
| 113 | E | A | -2.8580 | |
| 114 | K | A | -2.6759 | |
| 115 | T | A | 0.0000 | |
| 116 | S | A | 0.0000 | |
| 117 | D | A | -1.1861 | |
| 118 | Q | A | -0.7390 | |
| 119 | I | A | 0.0000 | |
| 120 | H | A | 0.0000 | |
| 121 | F | A | 0.0000 | |
| 122 | F | A | 0.0000 | |
| 123 | F | A | 0.0000 | |
| 124 | A | A | 0.0000 | |
| 125 | K | A | -1.2612 | |
| 126 | L | A | 0.0000 | |
| 127 | N | A | -1.0435 | |
| 128 | C | A | -1.7954 | |
| 129 | R | A | -2.6723 | |
| 130 | L | A | 0.0000 | |
| 131 | Y | A | -1.5248 | |
| 132 | R | A | -3.1016 | |
| 133 | K | A | -3.3922 | |
| 134 | A | A | -2.2569 | |
| 135 | N | A | -2.7364 | |
| 136 | K | A | -2.7193 | |
| 137 | S | A | -1.6091 | |
| 138 | S | A | -2.1557 | |
| 139 | K | A | -1.6685 | |
| 140 | L | A | -0.5054 | |
| 141 | V | A | -0.1541 | |
| 142 | S | A | 0.0870 | |
| 143 | A | A | 0.0000 | |
| 144 | N | A | -0.3284 | |
| 145 | R | A | -0.4533 | |
| 146 | L | A | 0.0000 | |
| 147 | F | A | 0.0000 | |
| 148 | G | A | 0.0000 | |
| 149 | D | A | 0.0000 | |
| 150 | K | A | -1.9479 | |
| 151 | S | A | -1.4510 | |
| 152 | L | A | -1.4683 | |
| 153 | T | A | -1.1318 | |
| 154 | F | A | -1.0687 | |
| 155 | N | A | -1.5551 | |
| 156 | E | A | -2.6074 | |
| 157 | T | A | -1.7498 | |
| 158 | Y | A | 0.0000 | |
| 159 | Q | A | -2.3835 | |
| 160 | D | A | -2.9356 | |
| 161 | I | A | -1.6280 | |
| 162 | S | A | 0.0000 | |
| 163 | E | A | -2.3245 | |
| 164 | L | A | -1.1804 | |
| 165 | V | A | 0.0000 | |
| 166 | Y | A | -0.8069 | |
| 167 | G | A | -1.5171 | |
| 168 | A | A | -1.2610 | |
| 169 | K | A | -1.7448 | |
| 170 | L | A | 0.0000 | |
| 171 | Q | A | -0.6313 | |
| 172 | P | A | -0.8247 | |
| 173 | L | A | -1.2980 | |
| 174 | D | A | -2.6082 | |
| 175 | F | A | 0.0000 | |
| 176 | K | A | -3.4284 | |
| 177 | E | A | -3.7030 | |
| 178 | N | A | -3.4064 | |
| 179 | A | A | 0.0000 | |
| 180 | E | A | -3.7683 | |
| 181 | Q | A | -2.6493 | |
| 182 | S | A | 0.0000 | |
| 183 | R | A | -2.4140 | |
| 184 | A | A | -1.8514 | |
| 185 | A | A | -1.2747 | |
| 186 | I | A | 0.0000 | |
| 187 | N | A | -1.7493 | |
| 188 | K | A | -2.4450 | |
| 189 | W | A | -1.4470 | |
| 190 | V | A | 0.0000 | |
| 191 | S | A | -2.7423 | |
| 192 | N | A | -3.1059 | |
| 193 | K | A | -2.3110 | |
| 194 | T | A | 0.0000 | |
| 195 | E | A | -3.1390 | |
| 196 | G | A | -2.8563 | |
| 197 | R | A | -2.6993 | |
| 198 | I | A | 0.0000 | |
| 199 | T | A | -1.9256 | |
| 200 | D | A | -1.9946 | |
| 201 | V | A | 0.0000 | |
| 202 | I | A | 0.0000 | |
| 203 | P | A | -1.3770 | |
| 204 | S | A | -1.8914 | |
| 205 | E | A | -2.6039 | |
| 206 | A | A | -1.6034 | |
| 207 | I | A | 0.0000 | |
| 208 | N | A | -3.0843 | |
| 209 | E | A | -3.2381 | |
| 210 | L | A | -0.3309 | |
| 211 | T | A | -0.4367 | |
| 212 | V | A | -0.5891 | |
| 213 | L | A | 0.0000 | |
| 214 | V | A | 0.0000 | |
| 215 | L | A | 0.0000 | |
| 216 | V | A | 0.0000 | |
| 217 | N | A | 0.0000 | |
| 218 | T | A | 0.0000 | |
| 219 | I | A | 0.0000 | |
| 220 | Y | A | 0.0000 | |
| 221 | F | A | 0.0000 | |
| 222 | K | A | -0.9073 | |
| 223 | G | A | 0.0000 | |
| 224 | L | A | -1.0686 | |
| 225 | W | A | 0.0000 | |
| 226 | K | A | -2.0210 | |
| 227 | S | A | -1.7809 | |
| 228 | K | A | -1.7674 | |
| 229 | F | A | 0.0000 | |
| 230 | S | A | -1.2746 | |
| 231 | P | A | -1.2335 | |
| 232 | E | A | -1.6344 | |
| 233 | N | A | -1.2271 | |
| 234 | T | A | 0.0000 | |
| 235 | R | A | -0.6136 | |
| 236 | K | A | -0.8100 | |
| 237 | E | A | -0.8660 | |
| 238 | L | A | 0.1043 | |
| 239 | F | A | 0.0000 | |
| 240 | Y | A | -0.2825 | |
| 241 | K | A | -1.5915 | |
| 242 | A | A | -2.0892 | |
| 243 | D | A | -2.8476 | |
| 244 | G | A | -2.1198 | |
| 245 | E | A | -2.5299 | |
| 246 | S | A | -1.0747 | |
| 247 | A | A | -0.0900 | mutated: CA247A |
| 248 | S | A | 0.1231 | |
| 249 | A | A | 0.0000 | |
| 250 | S | A | -0.2178 | |
| 251 | M | A | 0.0000 | |
| 252 | M | A | 0.0000 | |
| 253 | Y | A | -1.0581 | |
| 254 | Q | A | -1.4830 | |
| 255 | E | A | -2.0870 | |
| 256 | G | A | -1.7789 | |
| 257 | K | A | -2.4023 | |
| 258 | F | A | 0.0000 | |
| 259 | R | A | -2.6676 | |
| 260 | Y | A | 0.0000 | |
| 261 | R | A | -2.5708 | |
| 262 | R | A | -2.5347 | |
| 263 | V | A | -1.5957 | |
| 264 | A | A | -1.1513 | |
| 265 | E | A | -2.3535 | |
| 266 | G | A | -2.1196 | |
| 267 | T | A | 0.0000 | |
| 268 | Q | A | -1.4594 | |
| 269 | V | A | 0.0000 | |
| 270 | L | A | 0.0000 | |
| 271 | E | A | 0.0000 | |
| 272 | L | A | 0.0000 | |
| 273 | P | A | -1.3021 | |
| 274 | F | A | 0.0000 | |
| 275 | K | A | -2.4712 | |
| 276 | G | A | -2.3214 | |
| 277 | D | A | -2.2661 | |
| 278 | D | A | -1.9578 | |
| 279 | I | A | 0.0000 | |
| 280 | T | A | 0.0000 | |
| 281 | M | A | 0.0000 | |
| 282 | V | A | 0.0000 | |
| 283 | L | A | 0.0000 | |
| 284 | I | A | 0.0000 | |
| 285 | L | A | 0.0000 | |
| 286 | P | A | 0.0000 | |
| 287 | K | A | -2.6788 | |
| 288 | P | A | -1.8898 | |
| 289 | E | A | -2.6005 | |
| 290 | K | A | -2.4607 | |
| 291 | S | A | -1.7006 | |
| 292 | L | A | 0.0000 | |
| 293 | A | A | -2.0429 | |
| 294 | K | A | -2.6277 | |
| 295 | V | A | 0.0000 | |
| 296 | E | A | 0.0000 | |
| 297 | K | A | -3.3249 | |
| 298 | E | A | -2.7663 | |
| 299 | L | A | 0.0000 | |
| 300 | T | A | -1.3030 | |
| 301 | P | A | -1.5257 | |
| 302 | E | A | -2.3371 | |
| 303 | V | A | -1.4570 | |
| 304 | L | A | 0.0000 | |
| 305 | Q | A | -2.5138 | |
| 306 | E | A | -3.2888 | |
| 307 | W | A | -2.4299 | |
| 308 | L | A | -2.4395 | |
| 309 | D | A | -3.5652 | |
| 310 | E | A | -3.9380 | |
| 311 | L | A | 0.0000 | |
| 312 | E | A | -3.7492 | |
| 313 | E | A | -3.1783 | |
| 314 | M | A | -1.3896 | |
| 315 | M | A | -0.8325 | |
| 316 | L | A | 0.0000 | |
| 317 | V | A | 0.0000 | |
| 318 | V | A | 0.0000 | |
| 319 | H | A | 0.0000 | |
| 320 | M | A | 0.0000 | |
| 321 | P | A | 0.0000 | |
| 322 | R | A | -0.3612 | |
| 323 | F | A | 0.0000 | |
| 324 | R | A | -1.4546 | |
| 325 | I | A | 0.0000 | |
| 326 | E | A | -2.3084 | |
| 327 | D | A | -1.7817 | |
| 328 | G | A | -1.3691 | |
| 329 | F | A | -0.8484 | |
| 330 | S | A | -0.8956 | |
| 331 | L | A | 0.0000 | |
| 332 | K | A | -1.6578 | |
| 333 | E | A | -2.6076 | |
| 334 | Q | A | -2.0431 | |
| 335 | L | A | 0.0000 | |
| 336 | Q | A | -1.6973 | |
| 337 | D | A | -2.5362 | |
| 338 | M | A | -1.3234 | |
| 339 | G | A | -0.9290 | |
| 340 | L | A | 0.0000 | |
| 341 | V | A | -0.3914 | |
| 342 | D | A | 0.0000 | |
| 343 | L | A | 0.0000 | |
| 344 | F | A | 0.0000 | |
| 345 | S | A | -1.3537 | |
| 346 | P | A | -2.2805 | |
| 347 | E | A | -3.3852 | |
| 348 | K | A | -3.3396 | |
| 349 | S | A | 0.0000 | |
| 350 | K | A | -2.5382 | |
| 351 | L | A | 0.0000 | |
| 352 | P | A | -0.6899 | |
| 353 | G | A | 0.0000 | |
| 354 | I | A | 0.0000 | |
| 355 | V | A | 0.0000 | |
| 356 | A | A | -1.7390 | |
| 357 | E | A | -3.4849 | |
| 358 | G | A | -2.7128 | |
| 359 | R | A | -3.6473 | |
| 360 | D | A | -4.4029 | |
| 361 | D | A | -4.1906 | |
| 362 | L | A | 0.0000 | |
| 363 | Y | A | -1.1437 | |
| 364 | V | A | 0.0000 | |
| 365 | S | A | 0.1284 | |
| 366 | D | A | -0.5427 | |
| 367 | A | A | 0.0000 | |
| 368 | F | A | 0.0000 | |
| 369 | H | A | 0.0000 | |
| 370 | K | A | -1.1306 | |
| 371 | A | A | 0.0000 | |
| 372 | F | A | -1.2608 | |
| 373 | L | A | 0.0000 | |
| 374 | E | A | -1.0048 | |
| 375 | V | A | 0.0000 | |
| 376 | N | A | 0.0000 | |
| 377 | E | A | 0.0000 | |
| 378 | E | A | -0.9026 | |
| 379 | G | A | 0.0000 | |
| 380 | S | A | 0.0000 | |
| 381 | E | A | 0.0000 | |
| 382 | A | A | -0.2216 | |
| 383 | A | A | -0.0152 | |
| 384 | A | A | -0.1339 | |
| 385 | S | A | 0.1463 | |
| 386 | T | A | 0.4859 | |
| 387 | A | A | 0.9371 | |
| 388 | V | A | 2.0897 | |
| 389 | V | A | 1.2757 | |
| 390 | I | A | 1.7058 | |
| 391 | A | A | 0.3436 | |
| 392 | G | A | -0.4459 | |
| 393 | R | A | -0.4844 | |
| 394 | S | A | 0.1264 | |
| 395 | L | A | 0.9654 | |
| 396 | N | A | -0.2089 | |
| 397 | P | A | -0.9139 | |
| 398 | N | A | -1.8434 | |
| 399 | R | A | -1.4706 | |
| 400 | V | A | 0.6705 | |
| 401 | T | A | 0.1240 | |
| 402 | F | A | 0.0000 | |
| 403 | K | A | -0.7278 | |
| 404 | A | A | 0.0000 | |
| 405 | N | A | -0.3347 | |
| 406 | R | A | -0.5898 | |
| 407 | P | A | -0.7156 | |
| 408 | F | A | 0.0000 | |
| 409 | L | A | 0.0000 | |
| 410 | V | A | 0.0000 | |
| 411 | F | A | 0.0000 | |
| 412 | I | A | 0.0000 | |
| 413 | R | A | 0.0000 | |
| 414 | E | A | 0.0000 | |
| 415 | V | A | -0.5131 | |
| 416 | P | A | -0.9159 | |
| 417 | L | A | 0.0000 | |
| 418 | N | A | -0.6014 | |
| 419 | T | A | 0.0000 | |
| 420 | I | A | 0.0000 | |
| 421 | I | A | 0.0000 | |
| 422 | F | A | 0.0000 | |
| 423 | M | A | 0.0000 | |
| 424 | G | A | 0.0000 | |
| 425 | R | A | 0.0000 | |
| 426 | V | A | 0.0000 | |
| 427 | A | A | 0.0000 | |
| 428 | N | A | -0.9306 | |
| 429 | P | A | -0.5425 | |
| 430 | A | A | 0.2966 | mutated: CA430A |
| 431 | V | A | 0.8477 | |
| 432 | K | A | -0.9413 |
ALA alanine
ARG arginine
ASN asparagine
ASP aspartic acid
CYS cysteine
GLN glutamine
GLU glutamic acid
GLY glycine
HIS histidine
ILE isoleucine
LEU leucine
LYS lysine
MET methionine
PHE phenylalanine
PRO proline
SER serine
THR threonine
TRP tryptophan
TYR tyrosine
VAL valine