Project name: SH3_K108P

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	KA108P
Energy difference between WT (input) and mutated protein (by FoldX)	1.00725 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1042
• Maximal score value: 1.9467
• Average score: -0.8991
• Total score value: -51.2496

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5409
86	L	A	1.0106
87	F	A	1.1933
88	V	A	0.8105
89	A	A	0.0000
90	L	A	-0.0590
91	Y	A	-0.5656
92	D	A	-2.5595
93	Y	A	-1.9356
94	E	A	-2.6500
95	A	A	-2.6306
96	R	A	-2.9870
97	T	A	-2.6622
98	E	A	-3.1042
99	D	A	-3.0446
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2177
103	F	A	0.0000
104	H	A	-2.6289
105	K	A	-2.2876
106	G	A	-1.2426
107	E	A	-0.9338
108	P	A	0.0986	mutated: KA108P
109	F	A	0.0000
110	Q	A	-0.3647
111	I	A	0.0238
112	L	A	0.1395
113	N	A	-0.8895
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0571
121	E	A	-1.1548
122	A	A	0.0000
123	R	A	-1.6303
124	S	A	0.0000
125	L	A	0.2174
126	T	A	-0.3520
127	T	A	-0.7697
128	G	A	-1.3858
129	E	A	-2.2557
130	T	A	-1.7052
131	G	A	-1.5008
132	Y	A	-0.8688
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1508
137	Y	A	-0.1157
138	V	A	0.0000
139	A	A	0.5256
140	P	A	0.8906
141	V	A	1.9467