Project name: 3BG3 pyruvate carboxylase chain A

Chain sequence(s)	A: QNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPVVPAVPIGPPPAGFRDILLREGPEGFARAVRNHPGLLLMDTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFSKLFSMENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYLPNMLLGMEAAGSAGGVVEAAISYTGDVADPSRTKYSLQYYMGLAEELVRAGTHILCIDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAQAGADVVDVAADSMSGMTSQPSMGALVACTRGTPLDTEVPMERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGVPHGGFPEPFRSKVLKDLPRVEGRPGASLPPLDLQALEKELVDRHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEKALKDVKGQIGAPMPGKVIDIKVVAGAKVAKGQPLCVLSAMKMETVVTSPMEGTVRKVHVTKDMTLEGDDLILEIE
Distance of aggregation	10 Å
Dynamic mode	No

• Minimal score value: -3.6374
• Maximal score value: 1.8498
• Average score: -0.7341
• Total score value: -498.454

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
494	Q	A	-2.6946
495	N	A	-3.0408
496	R	A	-3.1740
497	A	A	0.0000
498	Q	A	-2.3305
499	K	A	-2.0178
500	L	A	0.0000
501	L	A	0.0000
502	H	A	-0.8760
503	Y	A	0.0000
504	L	A	0.0000
505	G	A	0.0000
506	H	A	-0.5571
507	V	A	0.0000
508	M	A	-0.0303
509	V	A	0.0000
510	N	A	-0.9034
511	G	A	-0.4255
512	P	A	-0.1538
513	T	A	-0.0696
514	T	A	0.0000
515	P	A	0.2271
516	I	A	0.3770
517	P	A	-0.0293
518	V	A	-0.4883
519	K	A	-1.4682
520	A	A	-0.7370
521	S	A	-0.6078
522	P	A	-0.5587
523	S	A	-0.6912
524	P	A	-0.7468
525	T	A	-0.8455
526	D	A	-1.3063
527	P	A	-0.7545
528	V	A	0.7297
529	V	A	0.6447
530	P	A	0.2602
531	A	A	0.9652
532	V	A	1.1812
533	P	A	0.9280
534	I	A	1.8498
535	G	A	0.5014
536	P	A	-0.1289
537	P	A	-0.1074
538	P	A	-0.4349
539	A	A	-0.3143
540	G	A	0.0000
541	F	A	0.0000
542	R	A	-0.4031
543	D	A	-0.5771
544	I	A	-0.6012
545	L	A	0.0000
546	L	A	0.0301
547	R	A	-1.9747
548	E	A	-2.8741
549	G	A	-1.6522
550	P	A	-1.7018
551	E	A	-2.7615
552	G	A	-2.3509
553	F	A	0.0000
554	A	A	-2.1512
555	R	A	-3.2071
556	A	A	-1.8479
557	V	A	0.0000
558	R	A	-2.1685
559	N	A	-2.3855
560	H	A	-1.3619
561	P	A	-0.9063
562	G	A	-1.0462
563	L	A	0.0000
564	L	A	0.0000
565	L	A	0.0000
566	M	A	0.0000
567	D	A	0.0000
568	T	A	0.0000
569	T	A	0.0000
570	F	A	0.0000
571	R	A	0.0000
572	D	A	0.0000
573	A	A	0.0000
574	H	A	0.0000
575	Q	A	-0.3223
576	S	A	0.0000
577	L	A	0.0000
578	L	A	0.0000
579	A	A	-0.2562
580	T	A	0.0000
581	R	A	0.0000
582	V	A	0.0000
583	R	A	0.0000
584	T	A	0.0000
585	H	A	-0.9519
586	D	A	0.0000
587	L	A	0.0000
588	K	A	-1.6264
589	K	A	-1.6646
590	I	A	0.0000
591	A	A	0.0000
592	P	A	-0.2972
593	Y	A	0.0000
594	V	A	0.0000
595	A	A	0.0000
596	H	A	-0.1578
597	N	A	-0.5569
598	F	A	0.0000
599	S	A	-0.6161
600	K	A	-1.2752
601	L	A	0.0000
602	F	A	0.0000
603	S	A	0.0000
604	M	A	0.0000
605	E	A	0.0000
606	N	A	0.0000
607	W	A	0.0000
608	G	A	0.0000
609	G	A	0.0000
610	A	A	-0.0271
611	T	A	0.0000
612	F	A	0.0000
613	D	A	0.0000
614	V	A	0.0000
615	A	A	0.0000
616	M	A	0.0000
617	R	A	-1.1195
618	F	A	-0.0348
619	L	A	0.0000
620	Y	A	-0.3087
621	E	A	0.0000
622	C	A	-0.3325
623	P	A	0.0000
624	W	A	0.0000
625	R	A	-1.0645
626	R	A	0.0000
627	L	A	0.0000
628	Q	A	-1.8677
629	E	A	-1.9397
630	L	A	0.0000
631	R	A	0.0000
632	E	A	-2.1056
633	L	A	-1.1426
634	I	A	0.0000
635	P	A	-0.7294
636	N	A	0.0000
637	I	A	0.0000
638	P	A	0.0000
639	F	A	0.0000
640	Q	A	0.0000
641	M	A	0.0000
642	L	A	0.1986
643	L	A	0.0000
644	R	A	0.0000
645	G	A	0.0000
646	A	A	0.0000
647	N	A	0.0000
648	A	A	0.0000
649	V	A	0.0000
650	G	A	0.0000
651	Y	A	0.3606
652	T	A	0.2912
653	N	A	0.0000
654	Y	A	0.1856
655	P	A	-0.2897
656	D	A	-0.4959
657	N	A	-0.6424
658	V	A	0.0000
659	V	A	0.0000
660	F	A	-0.6803
661	K	A	-0.9793
662	F	A	0.0000
663	C	A	0.0000
664	E	A	-2.1952
665	V	A	-1.6432
666	A	A	0.0000
667	K	A	-2.3554
668	E	A	-2.7303
669	N	A	-1.8577
670	G	A	-1.4225
671	M	A	0.0000
672	D	A	0.0000
673	V	A	0.0000
674	F	A	0.0000
675	R	A	0.0000
676	V	A	0.0000
677	F	A	0.0000
678	D	A	0.0000
679	S	A	0.0000
680	L	A	0.0000
681	N	A	0.0000
682	Y	A	0.2488
683	L	A	0.1484
684	P	A	0.1240
685	N	A	0.0000
686	M	A	0.0000
687	L	A	-0.1647
688	L	A	0.0000
689	G	A	0.0000
690	M	A	0.0000
691	E	A	-1.8519
692	A	A	0.0000
693	A	A	0.0000
694	G	A	-1.3025
695	S	A	-1.1520
696	A	A	-1.5625
697	G	A	-1.2621
698	G	A	0.0000
699	V	A	0.0000
700	V	A	0.0000
701	E	A	0.0000
702	A	A	0.0000
703	A	A	0.0000
704	I	A	0.0000
705	S	A	0.0000
706	Y	A	0.0000
707	T	A	0.0000
708	G	A	-1.0489
709	D	A	-1.0145
710	V	A	0.0000
711	A	A	-0.6507
712	D	A	-1.2568
713	P	A	-1.1664
714	S	A	-1.5172
715	R	A	-2.4791
716	T	A	-1.6424
717	K	A	-1.6704
718	Y	A	0.0000
719	S	A	-1.0664
720	L	A	-0.3532
721	Q	A	-1.0822
722	Y	A	-0.5668
723	Y	A	0.0000
724	M	A	-1.0558
725	G	A	-1.1889
726	L	A	0.0000
727	A	A	0.0000
728	E	A	-2.5749
729	E	A	-2.3532
730	L	A	0.0000
731	V	A	-1.5072
732	R	A	-2.5736
733	A	A	-1.5618
734	G	A	-1.4613
735	T	A	-1.0079
736	H	A	-0.9202
737	I	A	0.0000
738	L	A	0.0000
739	C	A	0.0000
740	I	A	0.0000
742	D	A	0.0000
743	M	A	0.0640
744	A	A	0.0000
745	G	A	0.0000
746	L	A	0.0000
747	L	A	0.0000
748	K	A	-0.7631
749	P	A	-0.2949
750	T	A	-0.2907
751	A	A	0.0000
752	C	A	0.0000
753	T	A	-0.2374
754	M	A	0.3430
755	L	A	0.0000
756	V	A	0.0000
757	S	A	-1.0050
758	S	A	-1.1243
759	L	A	0.0000
760	R	A	-2.0811
761	D	A	-3.0783
762	R	A	-3.1408
763	F	A	-2.1124
764	P	A	-2.1651
765	D	A	-2.8409
766	L	A	-1.6769
767	P	A	0.0000
768	L	A	0.0000
769	H	A	0.0000
770	I	A	0.0000
771	H	A	0.0000
772	T	A	0.0000
773	H	A	0.0000
774	D	A	-0.4046
775	T	A	0.0000
776	S	A	-0.6886
777	G	A	-0.7712
778	A	A	-0.3935
779	G	A	0.0000
780	V	A	0.3575
781	A	A	0.1767
782	A	A	0.0000
783	M	A	0.0000
784	L	A	0.2652
785	A	A	-0.3201
786	C	A	0.0000
787	A	A	0.0000
788	Q	A	-1.1554
789	A	A	-0.6374
790	G	A	-0.6344
791	A	A	0.0000
792	D	A	0.0000
793	V	A	0.0000
794	V	A	0.0000
795	D	A	0.0000
796	V	A	0.0000
797	A	A	0.0000
798	A	A	0.0000
799	D	A	-1.1069
800	S	A	-1.2920
801	M	A	0.0000
802	S	A	-0.3786
803	G	A	-0.3639
804	M	A	-0.6004
805	T	A	0.0000
806	S	A	0.0000
807	Q	A	0.0000
808	P	A	0.0000
809	S	A	-0.3154
810	M	A	0.0000
811	G	A	0.0000
812	A	A	0.2044
813	L	A	0.0000
814	V	A	0.0000
815	A	A	-0.4828
816	C	A	0.1530
817	T	A	0.0000
818	R	A	-2.3250
819	G	A	-1.5601
820	T	A	-0.9331
821	P	A	-0.8989
822	L	A	-0.9659
823	D	A	-1.7438
824	T	A	0.0000
825	E	A	-2.4736
826	V	A	0.0000
827	P	A	-1.3038
828	M	A	-0.9703
829	E	A	-1.8594
830	R	A	-1.7709
831	V	A	0.0000
832	F	A	-0.2611
833	D	A	-1.2982
834	Y	A	0.0000
835	S	A	-1.5310
836	E	A	-2.1534
837	Y	A	0.0000
838	W	A	0.0000
839	E	A	-2.6351
840	G	A	-1.5052
841	A	A	0.0000
842	R	A	-1.1715
843	G	A	-1.1212
844	L	A	0.0000
845	Y	A	0.0000
846	A	A	-0.3091
847	A	A	0.0000
848	F	A	0.0000
849	D	A	-0.2019
850	C	A	0.0000
851	T	A	-0.6181
852	A	A	-0.5173
853	T	A	-0.6522
854	M	A	0.0000
855	K	A	-1.8874
856	S	A	-1.3448
857	G	A	-1.1306
858	N	A	-1.1253
859	S	A	-0.9873
860	D	A	-1.0899
861	V	A	0.0000
862	Y	A	-0.6473
863	E	A	-1.8505
864	N	A	0.0000
865	E	A	-0.5644
866	I	A	0.0000
867	P	A	0.0000
868	G	A	0.0000
869	G	A	-0.4056
870	Q	A	-0.5817
871	Y	A	0.0000
872	T	A	0.0000
873	N	A	-0.9982
874	L	A	0.0000
875	H	A	-1.1116
876	F	A	-0.4489
877	Q	A	-1.0819
878	A	A	0.0000
879	H	A	-0.1523
880	S	A	0.1151
881	M	A	0.8138
882	G	A	0.2140
883	L	A	0.8553
884	G	A	-0.3629
885	S	A	-1.5705
886	K	A	-2.5928
887	F	A	0.0000
888	K	A	-3.1988
889	E	A	-3.0535
890	V	A	0.0000
891	K	A	-2.3882
892	K	A	-2.6601
893	A	A	-1.6133
894	Y	A	0.0000
895	V	A	-1.3471
896	E	A	-1.4900
897	A	A	0.0000
898	N	A	0.0000
899	Q	A	-1.5115
900	M	A	0.0000
901	L	A	0.0000
902	G	A	-1.3026
903	D	A	-1.5359
904	L	A	0.0000
905	I	A	0.0000
906	K	A	0.0000
907	V	A	0.0071
908	T	A	-0.0528
909	P	A	0.0000
910	S	A	0.0000
911	S	A	0.0000
912	K	A	-0.8125
913	I	A	0.0000
914	V	A	0.0000
915	G	A	0.0000
916	D	A	-1.0990
917	L	A	0.0000
918	A	A	0.0000
919	Q	A	-1.0448
920	F	A	-0.7398
921	M	A	0.0000
922	V	A	-1.4237
923	Q	A	-1.7287
924	N	A	-1.8976
925	G	A	-1.4325
926	L	A	-1.2356
927	S	A	-1.3736
928	R	A	-1.4760
929	A	A	-1.4053
930	E	A	-2.2095
931	A	A	0.0000
932	E	A	-1.9082
933	A	A	-1.8582
934	Q	A	-2.3818
935	A	A	0.0000
936	E	A	-3.4370
937	E	A	-2.8597
938	L	A	-1.4777
939	S	A	-1.1384
940	F	A	0.0000
941	P	A	-1.1465
942	R	A	-2.1115
943	S	A	-1.2302
944	V	A	0.0000
945	V	A	-0.6371
946	E	A	-1.1048
947	F	A	0.0000
948	L	A	0.0000
949	Q	A	-0.9668
950	G	A	0.0000
951	Y	A	-0.0289
952	I	A	0.0000
953	G	A	0.0000
954	V	A	0.1387
955	P	A	0.0000
956	H	A	-0.7273
957	G	A	-0.9114
958	G	A	-0.7062
959	F	A	-0.7252
960	P	A	-1.2622
961	E	A	-1.8646
962	P	A	-1.2955
963	F	A	0.0000
964	R	A	-1.5360
965	S	A	-1.8910
966	K	A	-2.7525
967	V	A	-1.8921
968	L	A	0.0000
969	K	A	-2.9227
970	D	A	-2.6487
971	L	A	-1.3633
972	P	A	-1.6803
973	R	A	-2.3495
974	V	A	-1.5352
975	E	A	-2.2412
976	G	A	-1.3063
977	R	A	-0.9102
978	P	A	0.0000
979	G	A	0.0000
980	A	A	-0.5332
981	S	A	-0.5112
982	L	A	-0.0941
983	P	A	-0.4429
984	P	A	-0.6169
985	L	A	-0.5254
986	D	A	-1.6349
987	L	A	-1.5765
988	Q	A	-2.5036
989	A	A	-2.0157
990	L	A	-1.9880
991	E	A	-2.8998
992	K	A	-3.5239
993	E	A	-3.4104
994	L	A	0.0000
995	V	A	-2.1892
996	D	A	-3.5116
997	R	A	-3.5153
998	H	A	-2.4993
999	G	A	-2.3143
1000	E	A	-2.9873
1001	E	A	-2.3745
1002	V	A	-1.1416
1003	T	A	-0.7645
1004	P	A	-0.6387
1005	E	A	-0.4204
1006	D	A	-0.5125
1007	V	A	0.0000
1008	L	A	0.0000
1009	S	A	0.0000
1010	A	A	0.0000
1011	A	A	-0.4339
1012	M	A	0.0000
1013	Y	A	0.0000
1014	P	A	-1.2500
1015	D	A	-2.3307
1016	V	A	0.0000
1017	F	A	0.0000
1018	A	A	-2.1851
1019	H	A	-2.3684
1020	F	A	0.0000
1021	K	A	-1.9586
1022	D	A	-2.4804
1023	F	A	0.0000
1024	T	A	-0.7791
1025	A	A	-1.0700
1026	T	A	-0.8561
1027	F	A	-0.6966
1028	G	A	0.0000
1029	P	A	-0.6644
1030	L	A	0.0000
1031	D	A	-0.6995
1032	S	A	-1.1101
1033	L	A	0.0000
1034	N	A	-0.7609
1035	T	A	0.0000
1036	R	A	-0.6907
1037	L	A	-0.3746
1038	F	A	0.0000
1039	L	A	0.0000
1040	Q	A	-0.6776
1041	G	A	0.0000
1042	P	A	0.0000
1043	K	A	-1.2619
1044	I	A	0.7803
1045	A	A	-0.3267
1046	E	A	-1.6632
1047	E	A	-2.9226
1048	F	A	0.0000
1049	E	A	-3.0114
1050	V	A	0.0000
1051	E	A	-3.6374
1052	L	A	-2.8221
1053	E	A	-3.4717
1054	R	A	-3.5275
1055	G	A	-3.0758
1056	K	A	-3.0338
1057	T	A	-2.5991
1058	L	A	0.0000
1059	H	A	-3.0517
1060	I	A	0.0000
1061	K	A	-1.8990
1062	A	A	0.0000
1063	L	A	0.5469
1064	A	A	0.5581
1065	V	A	0.5751
1066	S	A	-0.7689
1067	D	A	-2.0402
1068	L	A	-1.6121
1069	N	A	-2.0911
1070	R	A	-2.2596
1071	A	A	-1.2987
1072	G	A	0.0000
1073	Q	A	-1.7531
1074	R	A	-1.5081
1075	Q	A	-0.7157
1076	V	A	0.0000
1077	F	A	0.5313
1078	F	A	0.0000
1079	E	A	-1.3624
1080	L	A	0.0000
1081	N	A	-2.2813
1082	G	A	-1.8013
1083	Q	A	-1.6300
1084	L	A	0.0936
1085	R	A	-0.6816
1086	S	A	0.0162
1087	I	A	0.0000
1088	L	A	0.1149
1089	V	A	0.0000
1090	K	A	-1.9696
1091	D	A	0.0000
1092	T	A	-1.6294
1093	Q	A	-2.0936
1094	A	A	-1.7615
1095	M	A	-1.7930
1096	K	A	-2.6430
1097	E	A	-2.6574
1103	K	A	-1.8087
1104	A	A	-0.7679
1105	L	A	-0.4947
1106	K	A	-1.5721
1107	D	A	-2.0238
1108	V	A	-0.3029
1109	K	A	-2.0069
1110	G	A	-1.7759
1111	Q	A	-1.5068
1112	I	A	-0.8174
1113	G	A	-1.3132
1114	A	A	0.0000
1115	P	A	-0.9494
1116	M	A	0.0000
1117	P	A	-1.5468
1118	G	A	0.0000
1119	K	A	-1.5950
1120	V	A	0.0000
1121	I	A	-0.0401
1122	D	A	-0.6187
1123	I	A	0.2528
1124	K	A	-0.5562
1125	V	A	0.0000
1126	V	A	1.1791
1127	A	A	-0.2568
1128	G	A	-1.2052
1129	A	A	-0.9003
1130	K	A	-2.2554
1131	V	A	0.0000
1132	A	A	-1.9096
1133	K	A	-2.3771
1134	G	A	-1.5619
1135	Q	A	-1.1690
1136	P	A	-0.2312
1137	L	A	0.0000
1138	C	A	0.0000
1139	V	A	0.2932
1140	L	A	0.0000
1141	S	A	-0.4718
1142	A	A	-0.3236
1143	M	A	0.0847
1144	K	A	-1.1675
1145	M	A	-0.4671
1146	E	A	-1.3226
1147	T	A	0.0249
1148	V	A	1.0094
1149	V	A	0.0000
1150	T	A	-0.1054
1151	S	A	0.0000
1152	P	A	-1.3197
1153	M	A	-1.3459
1154	E	A	-2.8242
1155	G	A	-2.3699
1156	T	A	-2.1195
1157	V	A	0.0000
1158	R	A	-3.3348
1159	K	A	-2.4849
1160	V	A	-0.8635
1161	H	A	-1.1468
1162	V	A	-0.7899
1163	T	A	-0.8802
1164	K	A	-2.2153
1165	D	A	-2.3622
1166	M	A	-0.9451
1167	T	A	-1.1886
1168	L	A	0.0000
1169	E	A	-2.5928
1170	G	A	-1.8087
1171	D	A	-2.5627
1172	D	A	0.0000
1173	L	A	-0.7289
1174	I	A	0.0000
1175	L	A	0.0000
1176	E	A	-1.9003
1177	I	A	-2.2322
1178	E	A	-2.7704