Project name: SH3_E107G

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	EA107G
Energy difference between WT (input) and mutated protein (by FoldX)	2.43254 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1049
• Maximal score value: 1.799
• Average score: -0.9664
• Total score value: -55.0854

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5161
86	L	A	0.7936
87	F	A	0.9536
88	V	A	0.4917
89	A	A	0.0000
90	L	A	-0.1024
91	Y	A	-0.5206
92	D	A	-2.4759
93	Y	A	-1.8684
94	E	A	-2.6024
95	A	A	-2.5987
96	R	A	-2.9879
97	T	A	-2.6628
98	E	A	-3.1049
99	D	A	-3.0452
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.1215
103	F	A	-1.7384
104	H	A	-2.5757
105	K	A	-2.3025
106	G	A	-1.3406
107	G	A	-1.0798	mutated: EA107G
108	K	A	-0.5198
109	F	A	0.0000
110	Q	A	-0.5093
111	I	A	-0.0552
112	L	A	0.1430
113	N	A	-0.8879
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0557
121	E	A	-1.1529
122	A	A	0.0000
123	R	A	-1.7170
124	S	A	0.0000
125	L	A	0.0840
126	T	A	-0.4105
127	T	A	-0.7823
128	G	A	-1.3624
129	E	A	-2.2229
130	T	A	-1.6962
131	G	A	-1.5015
132	Y	A	-0.8695
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1504
137	Y	A	-0.1031
138	V	A	0.0000
139	A	A	0.4178
140	P	A	0.7774
141	V	A	1.7990