Project name: SH3_L86P

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	LA86P
Energy difference between WT (input) and mutated protein (by FoldX)	1.03243 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.6158
• Average score: -1.0091
• Total score value: -57.5202

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.1316
86	P	A	0.0513	mutated: LA86P
87	F	A	0.5185
88	V	A	0.2292
89	A	A	0.0000
90	L	A	-0.1137
91	Y	A	-0.5342
92	D	A	-2.5196
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0436
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2061
103	F	A	0.0000
104	H	A	-2.6770
105	K	A	-2.3026
106	G	A	-1.2399
107	E	A	-1.1902
108	K	A	-0.8483
109	F	A	0.0000
110	Q	A	-1.1858
111	I	A	-0.4007
112	L	A	0.0874
113	N	A	-0.9142
114	S	A	-1.1623
115	S	A	-1.5884
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0909
121	E	A	-1.1948
122	A	A	0.0000
123	R	A	-1.8499
124	S	A	0.0000
125	L	A	0.1072
126	T	A	-0.3315
127	T	A	-0.7516
128	G	A	-1.3336
129	E	A	-2.1920
130	T	A	-1.7069
131	G	A	-1.4850
132	Y	A	-0.8552
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9195
136	N	A	-1.1504
137	Y	A	-0.1371
138	V	A	0.0000
139	A	A	0.3242
140	P	A	0.5321
141	V	A	1.6158