Project name: SH3_L125T

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	LA125T
Energy difference between WT (input) and mutated protein (by FoldX)	1.16948 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.8241
• Average score: -1.0401
• Total score value: -59.2857

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5522
86	L	A	0.7258
87	F	A	0.8142
88	V	A	0.2654
89	A	A	0.0000
90	L	A	-0.1681
91	Y	A	-0.5842
92	D	A	-2.5601
93	Y	A	-1.9321
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7316
105	K	A	-2.4135
106	G	A	-1.4672
107	E	A	-1.5592
108	K	A	-1.0179
109	F	A	0.0000
110	Q	A	-0.8325
111	I	A	-0.0843
112	L	A	0.1117
113	N	A	-0.9007
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0689
121	E	A	-1.1717
122	A	A	0.0000
123	R	A	-2.0518
124	S	A	0.0000
125	T	A	-1.1361	mutated: LA125T
126	T	A	-1.0584
127	T	A	-1.1639
128	G	A	-1.7358
129	E	A	-2.4360
130	T	A	-1.8446
131	G	A	-1.5033
132	Y	A	-0.8698
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1511
137	Y	A	-0.1359
138	V	A	0.0000
139	A	A	0.4174
140	P	A	0.7887
141	V	A	1.8241