Project name: SH3_I111L

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	IA111L
Energy difference between WT (input) and mutated protein (by FoldX)	2.20197 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7894
• Average score: -0.9527
• Total score value: -54.3014

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5269
86	L	A	0.8019
87	F	A	0.9198
88	V	A	0.4212
89	A	A	0.0000
90	L	A	-0.1536
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4606
107	E	A	-1.3110
108	K	A	-0.6399
109	F	A	0.0000
110	Q	A	-0.4757
111	L	A	0.0281	mutated: IA111L
112	L	A	0.1828
113	N	A	-0.8473
114	S	A	-1.1162
115	S	A	-1.5745
116	E	A	-2.5460
117	G	A	-2.1224
118	D	A	-2.4349
119	W	A	-1.0949
120	W	A	-1.0249
121	E	A	-1.1255
122	A	A	0.0000
123	R	A	-1.6978
124	S	A	0.0000
125	L	A	0.0570
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6843
131	G	A	-1.4929
132	Y	A	-0.8558
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9075
136	N	A	-1.1510
137	Y	A	-0.1267
138	V	A	0.0000
139	A	A	0.4087
140	P	A	0.7667
141	V	A	1.7894