Project name: SH3_F87I

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	FA87I
Energy difference between WT (input) and mutated protein (by FoldX)	4.59582 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7178
• Average score: -0.9769
• Total score value: -55.6836

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.4872
86	L	A	0.7131
87	I	A	0.0000	mutated: FA87I
88	V	A	0.3190
89	A	A	0.0000
90	L	A	-0.1774
91	Y	A	-0.5743
92	D	A	-2.5596
93	Y	A	-1.9312
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4057
106	G	A	-1.4855
107	E	A	-1.3428
108	K	A	-0.6989
109	F	A	0.0000
110	Q	A	-0.4871
111	I	A	0.0562
112	L	A	0.2291
113	N	A	-0.8328
114	S	A	-1.1384
115	S	A	-1.5921
116	E	A	-2.5537
117	G	A	-2.1270
118	D	A	-2.4341
119	W	A	-1.0819
120	W	A	-0.9792
121	E	A	-1.0912
122	A	A	0.0000
123	R	A	-1.6737
124	S	A	0.0000
125	L	A	0.0353
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6747
131	G	A	-1.4759
132	Y	A	-0.8361
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9216
136	N	A	-1.1445
137	Y	A	-0.1489
138	V	A	0.0000
139	A	A	0.3389
140	P	A	0.6765
141	V	A	1.7178