Project name: SH3_E121L

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	EA121L
Energy difference between WT (input) and mutated protein (by FoldX)	-2.15979 kcal/mol Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.0977
• Maximal score value: 1.7964
• Average score: -0.8798
• Total score value: -50.146

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9238
88	V	A	0.4313
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6208
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.0977
99	D	A	-2.9305
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2004
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3078
108	K	A	-0.6426
109	F	A	0.0000
110	Q	A	-0.3954
111	I	A	0.1755
112	L	A	0.4203
113	N	A	-0.5275
114	S	A	-0.9560
115	S	A	-1.4832
116	E	A	-2.4268
117	G	A	-2.0365
118	D	A	-2.3364
119	W	A	-0.8788
120	W	A	-0.6007
121	L	A	-0.2576	mutated: EA121L
122	A	A	0.0000
123	R	A	-1.5385
124	S	A	0.0000
125	L	A	0.0595
126	T	A	-0.4730
127	T	A	-0.8202
128	G	A	-1.3558
129	E	A	-2.2373
130	T	A	-1.4924
131	G	A	-1.1929
132	Y	A	-0.4621
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.8085
136	N	A	-1.1504
137	Y	A	-0.1243
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7757
141	V	A	1.7964