Project name: SH3_E107D

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	EA107D
Energy difference between WT (input) and mutated protein (by FoldX)	-0.0460258 kcal/mol Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1049
• Maximal score value: 1.7964
• Average score: -0.9637
• Total score value: -54.9321

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9155
88	V	A	0.4144
89	A	A	0.0000
90	L	A	-0.1592
91	Y	A	-0.5817
92	D	A	-2.5704
93	Y	A	-1.9372
94	E	A	-2.6525
95	A	A	-2.6330
96	R	A	-2.9880
97	T	A	-2.6629
98	E	A	-3.1049
99	D	A	-3.0453
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2213
103	F	A	0.0000
104	H	A	-2.7483
105	K	A	-2.4228
106	G	A	-1.4843
107	D	A	-1.3620	mutated: EA107D
108	K	A	-0.6689
109	F	A	0.0000
110	Q	A	-0.5091
111	I	A	-0.0568
112	L	A	0.1429
113	N	A	-0.8879
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0557
121	E	A	-1.1530
122	A	A	0.0000
123	R	A	-1.7095
124	S	A	0.0000
125	L	A	0.0509
126	T	A	-0.4832
127	T	A	-0.8208
128	G	A	-1.3480
129	E	A	-2.2233
130	T	A	-1.6852
131	G	A	-1.4974
132	Y	A	-0.8696
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1504
137	Y	A	-0.1243
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7757
141	V	A	1.7964