Project name: SH3_L125N

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	LA125N
Energy difference between WT (input) and mutated protein (by FoldX)	1.88799 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7965
• Average score: -1.1257
• Total score value: -64.1672

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.4916
86	L	A	0.4461
87	F	A	0.6448
88	V	A	0.1546
89	A	A	0.0000
90	L	A	-0.1512
91	Y	A	-0.5750
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7279
105	K	A	-2.4057
106	G	A	-1.4577
107	E	A	-1.8027
108	K	A	-1.4397
109	F	A	0.0000
110	Q	A	-1.1474
111	I	A	-0.1250
112	L	A	0.1062
113	N	A	-0.9021
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0714
121	E	A	-1.1759
122	A	A	0.0000
123	R	A	-2.3885
124	S	A	0.0000
125	N	A	-2.3614	mutated: LA125N
126	T	A	-1.6637
127	T	A	-1.5163
128	G	A	-2.1066
129	E	A	-2.6328
130	T	A	-1.9841
131	G	A	-1.5061
132	Y	A	-0.8716
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1504
137	Y	A	-0.1246
138	V	A	0.0000
139	A	A	0.4157
140	P	A	0.7630
141	V	A	1.7965