Project name: SH3_T127G

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	TA127G
Energy difference between WT (input) and mutated protein (by FoldX)	0.375248 kcal/mol Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1043
• Maximal score value: 1.7978
• Average score: -0.9841
• Total score value: -56.0946

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7959
87	F	A	0.9200
88	V	A	0.4211
89	A	A	0.0000
90	L	A	-0.1571
91	Y	A	-0.5806
92	D	A	-2.5684
93	Y	A	-1.9355
94	E	A	-2.6503
95	A	A	-2.6308
96	R	A	-2.9871
97	T	A	-2.6623
98	E	A	-3.1043
99	D	A	-3.0439
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2176
103	F	A	0.0000
104	H	A	-2.7437
105	K	A	-2.4191
106	G	A	-1.4782
107	E	A	-1.3941
108	K	A	-0.6953
109	F	A	0.0000
110	Q	A	-0.5054
111	I	A	-0.0552
112	L	A	0.1437
113	N	A	-0.8883
114	S	A	-1.1799
115	S	A	-1.5966
116	E	A	-2.5603
117	G	A	-2.1347
118	D	A	-2.4456
119	W	A	-1.1053
120	W	A	-1.0569
121	E	A	-1.1522
122	A	A	0.0000
123	R	A	-1.7735
124	S	A	0.0000
125	L	A	-0.0557
126	T	A	-0.6703
127	G	A	-1.2181	mutated: TA127G
128	G	A	-1.5476
129	E	A	-2.3656
130	T	A	-1.7409
131	G	A	-1.4983
132	Y	A	-0.8684
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9214
136	N	A	-1.1508
137	Y	A	-0.1246
138	V	A	0.0000
139	A	A	0.4168
140	P	A	0.7767
141	V	A	1.7978